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Multiparametric flow cytometry of normal human bone marrow:

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Presentation on theme: "Multiparametric flow cytometry of normal human bone marrow:"— Presentation transcript:

1 Multiparametric flow cytometry of normal human bone marrow:
analysis and display strategies GEIL-GTLLF 2008 Part one : Leukocyte subsets A colour code is applied trhoughout this atlas: Granulocytes in red Monocytes in green Lymphocytes in purple All other cells, in a region of maturation defined by the exclusion of mature cell types and thus dubbed « bermudes » in cyan I.1

2 Combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 7 : HLADR/CD117/CD34/CD45 CD11b/CD14 p. I-5 HLADR/CD117 p. I.29 CD11b/CD16 p. I-6 DR/CD34 p. I.30 CD14/CD16 p. I-7 CD117/CD34 p. I.31 Tube 2 :CD11b/CD117/CD34/CD45 Tube 8 : CD34/CD56/CD19/CD45 CD11b/CD117 p. I-9 CD34/CD56 p. I.33 CD11b/CD34 p. I-10 CD34/CD19 p. I.34 CD117/CD34 p. I-11 CD56/CD19 p. I.35 Tube 3 :CD36/CD71/CD11c/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD36/CD71 p. I-13 CD64/CD24 p. I.37 CD36/CD11c p. I-14 CD64/CD10 p. I.38 CD71/CD11c p. I-15 CD24/CD10 p. I.39 Tube 4 : CD34/CD13/CD33/CD45 Tube 10 : CD7/CD117/CD34/CD45 CD34/CD13 p. I-17 CD7/CD117 p. I.41 CD34/CD33 p. I.18 CD7/CD34 p. I.42 CD13/CD33 p. I.19 CD117/CD34 p. I.43 Tube 5 : CD38/HLADR/CD34/CD45 Tube 11 : CD22/CD10/CD20/CD45 CD38/HLADR p. I.21 CD22/CD10 p. I.45 CD38/CD34 p. I.22 CD22/CD20 p. I.46 HLADR/CD34 p. I.23 CD10/CD20 p. I.47 Tube 6 : CD38/CD10/CD19/CD45 Tube 12 : CD4/CD2/CD56/CD45 CD38/CD10 p. I.25 CD4/CD2 p. I.49 CD38/CD19 p. I.26 CD4/CD56 p. I.50 CD10/CD19 p. I.27 CD2/CD56 p. I.51 I.2

3 Leukocyte subsets display
FSC SSC CD45 SSC FLx FLy FLx FLy FLx FLy FLy FLx I.3

4 CD11b/CD14/CD16/CD45 TUBE 1 I.4 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD11b/CD14 x  x CD11b/CD16 x  CD14/CD16 I.4

5 CD11b/CD14 I.5 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC
SSC-Height CD14PE FLx FLy CD45 SSC FSC

6 CD11b/CD16 I.6 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC
SSC-Height CD16PC7 FLx FLy CD45 SSC FSC

7 CD14/CD16 I.7 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD14PE
SSC-Height CD16PC7 FLx FLy CD45 SSC FSC

8 CD11b/CD117/CD34/CD45 TUBE 2 I.8 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD11b/CD117 x x  CD11b/CD34 CD117/CD34 I.8

9 CD11b/CD117 I.9 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC
SSC-Height CD117PE FLx FLy CD45 SSC FSC

10 CD11b/CD34 I.10 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC
SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

11 CD117/CD34 I.11 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD117PE
SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

12 CD36/CD71/CD11c/CD45 TUBE 3 I.12 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD 36/CD71 x CD36/CD11c x  CD71/CD11c I.12

13 CD36/CD71 I.13 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD36FITC
SSC-Height CD71PE FLx FLy CD45 SSC FSC

14 CD36/CD11c I.14 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD36FITC
SSC-Height CD11cPC5 FLx FLy CD45 SSC FSC

15 CD71/CD11c I.15 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD71PE
SSC-Height CD11cPC5 FLx FLy CD45 SSC FSC

16 CD34/CD13/CD33/CD45 TUBE 4 I.16 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD34/CD13 x  x CD34/CD33 CD13/CD33 I.16

17 CD34/CD13 I.17 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD34FITC
SSC-Height CD13PE FLx FLy CD45 SSC FSC

18 CD34/CD33 I.18 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD34FITC
SSC-Height CD33APC FLx FLy CD45 SSC FSC

19 CD13/CD33 I.19 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD13PE
SSC-Height CD33APC FLx FLy CD45 SSC FSC

20 CD38/HLADR/CD34/CD45 TUBE 5 I.20 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD38/DR x x  CD38/CD34 DR/CD34 I.20

21 CD38/HLADR I.21 SSC FSC CD45 FLy FLx CD45ECD FSC-Height CD38FITC
SSC-Height HLADRPE FLx FLy CD45 SSC FSC

22 CD38/CD34 I.22 SSC FSC CD45 FLy FLx CD45ECD FSC-Height CD38FITC
SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

23 HLADR/CD34 I.23 SSC FSC CD45 FLy FLx CD45ECD FSC-Height HLADRPE
SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

24 CD38/CD10/CD19/CD45 TUBE 6 I.24 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD38/CD10 x x  CD38/CD19  x CD10/CD19 I.24

25 CD38/CD10 I.25 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD38FITC
SSC-Height CD10PE FLx FLy CD45 SSC FSC

26 CD38/CD19 I.26 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD38FITC
SSC-Height CD19Cy5 FLx FLy CD45 SSC FSC

27 CD19/CD10 I.27 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD10PE
SSC-Height CD19Cy5 FLx FLy CD45 SSC FSC

28 HLADR/CD117/CD34/CD45 TUBE 7 I.28 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage DR/CD117 x DR/CD34 x  CD117/CD34  x I.28

29 HLADR/CD117 I.29 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height HLADRFITC
SSC-Height CD117PE FLx FLy CD45 SSC FSC

30 HLADR/CD34 I.30 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height HLADRFITC
SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

31 CD117/CD34 I.31 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD117PE
SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

32 CD34/CD56/CD19/CD45 TUBE 8 I.32 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD34/CD56 x  x CD34/CD19 x  CD56/CD19 I.32

33 CD34/CD56 I.33 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD34FITC
SSC-Height CD56PE FLx FLy CD45 SSC FSC

34 CD34/CD19 I.34 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD34FITC
SSC-Height CD19PC7 FLx FLy CD45 SSC FSC

35 CD56/CD19 I.35 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD56PE
SSC-Height CD19PC7 FLx FLy CD45 SSC FSC

36 CD64/CD24/CD10/CD45 TUBE 9 I.36 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD64/CD24 x  x CD64/CD10 x  CD24CD10 I.36

37 CD64/CD24 I.37 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD64FITC
SSC-Height CD24PE FLx FLy CD45 SSC FSC

38 CD64/CD10 I.38 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD64FITC
SSC-Height CD10Cy5 FLx FLy CD45 SSC FSC

39 CD24/CD10 I.39 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD24PE
SSC-Height CD10Cy5 FLx FLy CD45 SSC FSC

40 CD7/CD117/CD34/CD45 TUBE 10 I.40 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD7/CD117 x x  CD7/CD34 CD117/CD34  x I.40

41 CD7/CD117 I.41 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD7FITC
SSC-Height CD117PE FLx FLy CD45 SSC FSC

42 CD7/CD34 I.42 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD7FITC
SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

43 CD117/CD34 I.43 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD117PE
SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

44 CD22/CD10/CD20/CD45 TUBE 11 I.44 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD22/CD10 x CD22/CD20 x  CD10/CD20 I.44

45 CD22/CD10 I.45 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD22FITC
SSC-Height CD10PE FLx FLy CD45 SSC FSC

46 CD22/CD20 I.46 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD22FITC
SSC-Height CD20PC5 FLx FLy CD45 SSC FSC

47 CD10/CD20 I.47 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD10PE
SSC-Height CD20PC5 FLx FLy CD45 SSC FSC

48 CD4/CD2/CD56/CD45 TUBE 12 I.48 Granulocytes Monocytes Lymphocytes
Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD4/CD2 x  CD4/CD56 CD2/CD56 x I.48

49 CD4/CD2 I.49 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD4FITC
SSC-Height CD2PE FLx FLy CD45 SSC FSC

50 CD4/CD56 I.50 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD4FITC
SSC-Height CD56PC7 FLx FLy CD45 SSC FSC CD4/CD56 I.50

51 CD2/CD56 I.51 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD2PE SSC-Height
CD56PC7 FLx FLy CD45 SSC FSC

52 Published on ELN website July 2008
Contributors Christine Arnoulet Marie Christine Béné Françoise Durrieu Jean Feuillard Chantal Fossat Bernard Husson Hélène Jouault Francis Lacombe Marc Maynadié Coordination Francis Lacombe & Marie Christine Béné

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