1. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 1 Prepare a Wet Mount Michael John Hughey, MD Former CAPT, MC, USNR

2. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 2 Obtain a Specimen Spatula Cotton-tipped Applicator Obtain directly or from inside curve of speculum

3. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 3 Place on Microscope Slide Use very small amount Too much makes the slide too thick and unreadable

4. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 4 Add NaCl and KOH One drop Mix well Two slides vs. one slide?

5. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 5 Add Coverslip Remove excess fluid so the coverslip is stable. Heat the KOH slide?

6. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 6 Microscopic Examination Start with low power Rarely need to go above medium power Focus up and down through the plane of the cells

7. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 7 Yeast Tangle of threads Single branch

8. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 8 Trichomonas About the same size as a white blood cell Smaller than a vaginal epithelial cell Violent motion

9. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 9 Bacterial Vaginosis Clue cells Vaginal epithelial cells studded with bacteria Smudged, granular appearance Indistinct borders Clue Cell Normal Vaginal Epithelial Cell

10. Military Obstetrics & Gynecology. Medical Education Division, Brookside Associates, Ltd. Copyright 2005, All Rights Reserved Slide 10