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Development of a Modular Peristaltic Microfluidic Pump and Valve System 1/30/2007 BME 273 Group 20: Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister
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Microfluidics Minimal reagent consumption Increased speed of reactions Study of biological phenomena at the single cell level
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Current Pumps at VIIBRE Harvard Pico Plus syringe pumps $2,000 / pump Limiting complexity of microfluidic devices
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Diagram of the System Nitrogen or Liquid Fluid Flow Pneumatic Valves Peristaltic Pump Christmas Tree Nanophysiometer
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Pneumatic Valves Two-layer PDMS device –Flow layer –Control layer Thin PDMS membrane deflects into the flow channel when the control channel is pressurized Unger et al. 2000
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4 pneumatic valves in series Control pressure 20-25 psi Flow channel dimensions –100 um wide, 10 um tall (round) or 5 um tall (rectangular) Control channel –Valve area (300 um by 300 um) Current Design of Peristaltic Pump
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Pump in Action
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Completed Work Safety, photolithography, and microfabrication training Initial estimates of flow rates are 5 nl/min – much too low Fabricated and tested devices with flow above and control below – membrane stuck to roof of flow channel Control Flow
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Current Status Will try changing “off” state of control line to vacuum Developing ways to increase flow rates – likely need at least 100 nl/min Multiphase parallel pumps are ready to be fabricated The fabrication of a second control box is about to begin
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Current Work Increase cross-sectional area of flow channel Vias to switch flow channel layer Characterize flow rates –Pulse-chase with bolus of fluorescent solution –Head to head vs. syringe pump Kartalov et al. 2006 Flow Groisman & Quake 2004 t = 0 t = d / v
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LabVIEW Interface Design an improved interface that allows for input of pump sequences - possibly using Excel Controls will include valve on/off, rate, time, and schedule
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Future Work Tesla valves or microfluidic rectifiers for anisotropic flow resistance Pumps at either end of device for push/pull Long-term testing of mechanical stability of pumps Sylgard 186 for mechanical properties instead of Sylgard 184 Bardell 2000 Groisman & Quake 2004
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