1. Role of Rho-A in Glaucoma Jasleen Kaur Narmada Jayasankar Vijetha Vemulapalli

2. Outline Open angle Glaucoma RhoA pathway and hypothesis CATPA

3. Open Angle Glaucoma It can be defined as damage to the Optic nerve due to Increase in Intra ocular pressure (IOP)

4. Anatomy of the angle

5. Trabecular meshwork Shields textbook of glaucoma-2005 Cornea Retina

6. Cell cytoskeleton Green: actin Red: mitochondria Blue: nucleus

7. TM Cell Contractility Aqueous humor regulation is done by TM cell contractility and re-alignment of pores. There are two pathways that regulate TM contractility. Ca dependent pathways And the Ca independent pathway. Rho-a protein is involved in the Ca independent pathway.

8. General Function of Rho-A Involved in the maintenance of tight junctions. Tight junctions are found in epithelial cells and all cells where there is a separation or demarcation into different regions. If the Rho-A pathway does not function properly in the eye, it causes Glaucoma.

9. Ca independent pathway GDI GAP GEF GTP GDP P RhoA-GDP Plasma membrane Contraction Relaxation MLC pMLC M20 MYPT1 PP1C  Active MLCP M20 MYPT1 PP1C  Inactive MLCP RhoA-GDPRhoA-GTPRho kinase

10. CATPA

12. Point Mutations

14. References 1. http://biology.kenyon.edu/BMB/Chime2/ 2002/rhoA/FRAMES/captxt.htmhttp://biology.kenyon.edu/BMB/Chime2/ 2. http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=165390 3. PDB, CATH, SCOP 4. http://ajpcell.physiology.org/cgi/content/full/275/3/C798 5.http: //www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1220893 6. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=14815 7. http://147.52.72.117/ijmm/2002/volume9/number6/563-570.pdf 8. http://apodaca2.dept-med.pitt.edu/PDFs/Rajasesekeran-TJs.pdf 9. www.ucl.ac.uk/ioo/research/greenwood.htm