1. Regulation of Gene Expression in Bacteria and Their Viruses
CHAPTER 10
Regulation of Gene Expression in Bacteria and Their Viruses
Copyright 2008 © W H Freeman and Company

2. CHAPTER OUTLINE 10.1 Gene regulation
10.2 Discovery of the lac system: negative control
10.3 Catabolite repression of the lac operon: positive control
10.4 Dual positive and negative control: the arabinose operon
10.5 Metabolic pathways and additional levels of regulation: attenuation
10.6 Bacteriophage life cycles: more regulators, complex operons
10.7 Alternative sigma factors regulate large sets of genes

3. Regulatory proteins control transcription
Figure 10-2

4. Allosteric effectors bind to regulatory proteins
Figure 10-3

5. Repressor protein controls the lac operon
Figure 10-4

6. Lactose is broken down into two sugars
Figure 10-5

7. The lac operon is transcribed only in the presence of lactose
Figure 10-6a

8. The lac operon is transcribed only in the presence of lactose
Figure 10-6b

9. Structure of IPTG
Figure 10-7

10. Table 10-1

11. Operators are cis-acting
Figure 10-8

12. Table 10-2

13. Repressors are trans-acting
Figure 10-9

14. Table 10-3

15. The repressor contains a lactose-binding site
Figure 10-10

16. RNA polymerase contacts the promoter at specific sequences
Figure 10-11

17. The operator is a specific DNA sequence
Figure 10-12

18. Glucose levels control the lac operon
Figure 10-13

19. Glucose levels control the lac operon
Figure 10-13a

20. Glucose levels control the lac operon
Figure 10-13b

21. Many DNA binding sites are symmetrical
Figure 10-14

22. Binding of CAP bends DNA
Figure 10-15

23. CAP and RNA polymerase bind next to each other
Figure 10-16

24. Negative and positive control of the lac operon
Figure 10-17

25. Negative and positive control of the lac operon
Figure 10-17a

26. Negative and positive control of the lac operon
Figure 10-17b

27. Negative and positive control of the lac operon
Figure 10-17c

28. Repression and activation compared
Figure 10-18

29. Repression and activation compared
Figure 10-18a

30. Repression and activation compared
Figure 10-18b

31. Map of the ara operon
Figure 10-19

32. AraC serves as an activator and as a repressor
Figure 10-20

33. Gene order in the trp operon corresponds to reaction order in
the biosynthetic pathway
Figure 10-21

34. The trp mRNA leader sequence contains an attenuator region
and two tryptophan codons
Figure 10-22

35. Abundant tryptophan attenuates transcription of the trp operon
Figure 10-23

36. Leader peptides of amino acid biosynthesis operons
Figure 10-24

37. The life cycle of bacteriophage 
Figure 10-25

38. Phage  genome is organized for coordinate control
Figure 10-26

39. The lysogenic-versus-lytic cycle is determined by repressor
occupancy on the OR operators
Figure 10-27

40. Helix-turn-helix is a common DNA-binding motif
Figure 10-28

41. Amino acid side chains determine the specificity of DNA binding
Figure 10-29

42.  factors control clusters of unlinked genes
Figure 10-30a

43.  factors control clusters of unlinked genes
Figure 10-30b