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Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the.

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Presentation on theme: "Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the."— Presentation transcript:

1 Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the initials on the antibiotic disk. 3.Draw a picture of the zone of inhibition around each circle 4.Measure the diameter of the zone of inhibition in mm and then record it on in the table. Antimicrobial agent Dose Diameter of zone of inhibition Ampicillin (AM) 10µg Clindamycin (CM) 2µg Erythromycin (E) 15µg Gentamicin (GM) 10µg Cefoxitin (FOX) 30µg Vancomycin (VA) 30µg

2 Identifying drug resistant bacteria from a nasal swab 1.Find the other people that worked on the same plate as you (1-5) 2.Enter all your values for the diameter of the zone of inhibition. 3.Find the average diameter. 4.Enter it on the table

3 Identifying drug resistant bacteria from a nasal swab Plate 1 Antimicrobial agent Dose Diameter of zone of inhibition Average diameter Ampicillin (AM) 10µg 1. 2. 3. 4. 5. Clindamycin (CM) 2µg 1. 2. 3. 4. 5. Erythromycin (E) 15µg 1. 2. 3. 4. 5. Gentamicin (GM) 10µg 1. 2. 3. 4. 5. Cefoxitin (FOX) 30µg 1. 2. 3. 4. 5. Vancomycin (VA) 30µg 1. 2. 3. 4. 5.

4 Identifying drug resistant bacteria from a nasal swab Plate 2 Antimicrobial agent Dose Diameter of zone of inhibition Average diameter Ampicillin (AM) 10µg 1. 2. 3. 4. 5. Clindamycin (CM) 2µg 1. 2. 3. 4. 5. Erythromycin (E) 15µg 1. 2. 3. 4. 5. Gentamicin (GM) 10µg 1. 2. 3. 4. 5. Cefoxitin (FOX) 30µg 1. 2. 3. 4. 5. Vancomycin (VA) 30µg 1. 2. 3. 4. 5.

5 Identifying drug resistant bacteria from a nasal swab Plate 3 Antimicrobial agent Dose Diameter of zone of inhibition Average diameter Ampicillin (AM) 10µg 1. 2. 3. 4. 5. Clindamycin (CM) 2µg 1. 2. 3. 4. 5. Erythromycin (E) 15µg 1. 2. 3. 4. 5. Gentamicin (GM) 10µg 1. 2. 3. 4. 5. Cefoxitin (FOX) 30µg 1. 2. 3. 4. 5. Vancomycin (VA) 30µg 1. 2. 3. 4. 5.

6 Identifying drug resistant bacteria from a nasal swab Plate 4 Antimicrobial agent Dose Diameter of zone of inhibition Average diameter Ampicillin (AM) 10µg 1. 2. 3. 4. 5. Clindamycin (CM) 2µg 1. 2. 3. 4. 5. Erythromycin (E) 15µg 1. 2. 3. 4. 5. Gentamicin (GM) 10µg 1. 2. 3. 4. 5. Cefoxitin (FOX) 30µg 1. 2. 3. 4. 5. Vancomycin (VA) 30µg 1. 2. 3. 4. 5.

7 Identifying drug resistant bacteria from a nasal swab Plate 5 Antimicrobial agent Dose Diameter of zone of inhibition Average diameter Ampicillin (AM) 10µg 1. 2. 3. 4. 5. Clindamycin (CM) 2µg 1. 2. 3. 4. 5. Erythromycin (E) 15µg 1. 2. 3. 4. 5. Gentamicin (GM) 10µg 1. 2. 3. 4. 5. Cefoxitin (FOX) 30µg 1. 2. 3. 4. 5. Vancomycin (VA) 30µg 1. 2. 3. 4. 5.

8 Is your bacteria drug resistant? (Plate1 - Staph Aureus) Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

9 Is your bacteria drug resistant? (Plate 2 - Staph Aureus) Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

10 Is your bacteria drug resistant? (Plate 3 - Staph. Aureus) Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

11 Is your bacteria drug resistant? (Plate 4 - Staph. epididimis) Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

12 Is your bacteria drug resistant? (Plate 5 - staph epididimis) Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

13 Is Staph Aureus drug resistant? Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

14 Is Staph Epididimis drug resistant? Antimicr obial agent Diam eter of zone of inhib ition ResistantIntermediateSusceptibleR, I or S Ampicillin (AM) 10µg ≤ 13 N/a ≥ 17 Clindamy cin (CM) 2µg ≤ 14 15-20 ≥ 21 Erythrom ycin (E) 15µg ≤ 13 14-22 ≥ 23 Gentamici n (GM) 10µg ≤ 12 13-14 ≥ 15 Cefoxitin (FOX) 30µg ≤ 21 N/a ≥ 22 Vancomy cin (VA) 30µg ≤ 9 10-11 ≥ 12

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