1. The effect of the mass transport on the albumin concentration (Fixed condition - flow rate 125 ㎕ /min) Dayun Yu Department of Physics Kangwon National University Expectation of experiment Under completely transport-limited conditions initial binding rates can be expected to increase in parallel with the albumin concentration. Concentration is experimental parameter that can be controlled to minimize mass transport effects.

2. Materials of experiment(Equipment) - Spreeta TM Evaluation Kit - Syringe pump - Syringe Condition of experiment - Fixed concentration - flow rate: 125  l/min. - Variable condition - albumin concentration: 50  g/ml, 25  g/ml, 5  g/ml Materials of experiment(Solution) - Human Serum Albumin(HAS) : Sample solution - Phosphate Buffered Saline(PBS) : Buffer solution - 1% TritonX-100 + 0.1N NaOH : Washing solution - Index matching liquid : Fixed gold wafer and Revised refractive index. - Deionized Water - Alcohol : Washing gold wafer Experiment Condition

3. Experiment Result

4. Discussion The initial binding rate is found to be proportional to albumin concentration, which is in good accordance with theoretical expectations. We found that the reaction was dependent on concentration.