1. A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC 2574549/

2. Simple component annotation Figure 1 localizes 2 proteins to the septum of B. subtilis

3. What proteins?

4. What GO term? “YycG and FtsZ co- localize to the septum” Septum can be at the cellular level or at the level of a tissue Qualifier: colocalizes_with

5. Evidence Experimental - Yes –Mutant – No –Based on direct physical interaction – No –Direct assay – Yes IDA

6. Additional annotations Figures 2-4 include evidence for annotations that are not allowed in CACAO (protein binding) Figure 5 supports annotation of another protein, YycF to GO:0009295 ! Nucleoid via similar localization experiments Figure 6 supports other annotations