1. Chapter 3: Non-Associative Learning and Memory From Mechanisms of Memory, second edition By J. David Sweatt, Ph.D.

2. Cytoskeletal Rearrangement in Synaptic Plasticity

3. 32 P Incorporation into protein or adenine nucleotides Time (hrs) 32 P added Steady State Figure 1 Hypothetical Graph of 32 P-PO 4

4. C B A Figure 2 AMPA Receptors

5. Figure 3 Isoelectic Point 3 10 Molecular Weight High (approx 200 kDa) Low (approx 5 kDa) Protein Turnover

6. Figure 4 Actin Turnover

7. Figure 5 Aplysia californica

8. Figure 6 Siphon-gill and tail-siphon withdrawal reflexes of Aplysia

9. Figure 7: C Short-term Sensitization of the gill-withdrawal reflex in Aplysia

10. Figure 8 Circuit Diagrams of the Siphon-gill

11. Figure 9 Heterosynaptic Facilitation of the Sensorimotor

12. Figure 10 Molecular events in Aplysia

13. Figure 11 Mechanisms of long-term memory formation

14. Substrates PO 4 Substrates Ubiquitin-mediated proteolysis Facilitation of Neurotransmitter release PDE cAMP AMP cAMP Shorter Term Longer Term Reg.Cat. Reg.Cat. Reg.Cat. Reg.Cat. Reg. Cat. Reg. Cat. Figure 12 PKA Altered Gene Expression and

15. Figure 13 Aplysia

16. Figure 14 Cellular basis of behavior

17. Figure 15 The Eskin/ Byrne model in Aplysia long-term facilitation

18. Figure 16 Injury-induced Spinal and Behavioral Sensitization

19. Figure 17 Anatomical Pain Pathways Cortex Spinal cord Midbrain Amygdala Medulla DRG Aδ-fiber C-fiber Pons Hypothalamus PAG Thalamus Ascending Pathways Descending Pathways Withdrawal Reflex End-organ tissue nociceptor terminals RVM Figure 17

20. Blue Box 1 Adenylyl Cyclase α γ β GTP GDP ATP cAMP Substrate PO 4 Reg Cat. Ion Channels “G s ” Protein Receptor cAMP-Dependent Protein Kinase (PKA) The cAMP System Receptor α γ β GTPGDP “G o ” Protein PLC IP 3 DAG Ca ++ PKC Substrate PO 4 PIP 2 The PLC System Coupling of Receptors to Intracellular Messengers

21. Blue Box 2 Potential Signal Transduction routes leading to MAPK Activation in Neurons GFR Grb2 Rap1 MEK p42 mapk Raf-1 B-Raf PKA PKC PKA AC Effectors Sos Ras

22. PO 4 ras GDPGTP GTP Hydrolysis GAPs GTPase Activating Proteins e.g. NF1, SynGAP GEFs Guanine Nucleotide Exchange Factor Proteins e.g. SOS, cAMP GEF, Ca 2+ /DAG GEF, ras GRF InactiveActive + + Blue Box 3 Regulation of RAS

23. Blue Box 4 Habituation and Synaptic Inhibition

24. Blue Box 6 Swimming ContactWithdrawal Preparation for swimming A B C Escape Swimming in Tritonia