1. Today House Keeping –RT-PCR result –Final report (manuscript style) C-value paradox.ppt Phylogeny, COI: snail species ID-ed fungal sequences

2. RT-PCT results snail 1 2 3 6 6 7 8 9 9 LBP/BPI Physalysin PGRP eIF2  group 1 2 3 5 6 7 8 9 10 housekeeping Experimental

3. PARASITES AND SNAIL BIOLOGY “identity, possibilities” phylogenetics “intentions” transcriptomics PCR rDNA/mito Bioanalyzer DNA-free, direct sequencing gel electrophoresis nanodrop spec Sequence ID (BLAST) editing Phylogenetics electrophoresis RT-PCR gel CTAB Trizol TA cloning, B/W screening M13 sequencing Primer design, walking Qiagen plasmid extraction Restriction digests DNA RNA GenBank submission + Sevilleta snails

4. Radix swinhoei?

9. C-value paradox.ppt

10. Physid snail species identification Physella acuta Based on alignment of experimentally obtained partial COI encoding sequences with sequences from popset 164430598 For phylogenetic tree construction using parsimony

11. Majority Rule

12. Extended

13. Majority Rule Extended

14. Majority Rule Extended Majority Rule