1. 15 N Tracking habitat and resource use for Dosidicus gigas: a stable isotope analysis in the Northern Humboldt Current System Juan Argüelles · Anne Lorrain · Yves Cherel · Michelle Graco · Ricardo Tafur · Ana Alegre · Pepe Espinoza · Anatolio Taipe · Patricia Ayón · Arnaud Bertrand

2. Introduction Some limitations: absence or minimal amount of food in cephalopods stomach differences in digestibility among preys selectivity rejection of a potential prey D. gigas diet Stomach content:

3. Introduction D. Gigas habitat

4. Introduction Stable isotope analysis (SIA) Stable isotopes of consumers reflect that of prey as well as the habitat of the individual. Nitrogen isotopes typically indicate trophic position, while carbon isotopes reflect variation in baseline producers or habitat. You are what you eat

5. The life history of D. gigas in Peruvian waters is same for all individuals? D. Gigas is a highly mobile in Peruvian waters? D. gigas carry out migrations along latitude or longitude in Peruvian waters? There is a systematic increase in trophic position with size in Peruvian waters ?. Questions:

6. Data and methods (sampling on board jigging fleet) 234 D. gigas (2.2 and 98.8 cm mantle length) obtained from 03.48°S to 18.31°S, and up to 813 km from the shelf break from the commercial jigging fleet and research vessels (2008-2010) Thirty-four samples of zooplankton (2008 using a Hensen net). 3421 stomachs of D. gigas (14.3 - 112.5 cm ML) collected from 05°S to 18°S, onboard the jigging fleet from 2004 to 2009. Micromass at Department of Marine Biology, La Rochelle, France.

7. Data and methods Potential relationships between dependent variables (δ 15 N and δ 13 C) and independent variables (latitude, ML, distance to shelf break were investigated using generalized additive models (software R) Classification and regression trees (CART, Brieman et al. 1984) were used to assess the relative importance of Year, latitude, size (ML) and DSB on squid δ 15 N and δ 13 C values

8. Results: Isotopes variability and habitat VariableSourcee.d.fFP% explained δ 13 CLat3.76837.780< 2 e-1634.40 Size1.00026.5007.10 e-077.80 DSB8.28312.8307.77 e-1534.43 Model < 2 e-1676.63 δ 15 NLat3.62959.419< 2 e-1645.33 Size8.7639.9453.46 e-1228.72 DSB2.8537.3664.19 e-056.15 Model < 2 e-1682.20 Statistical outputs of GAMs based on mantle δ13C and δ15N values of D. gigas

9. Results:  13 C vs DSB  13 C Coastal waters (>productivity) Oceanic waters (<productivity)

10. Results:  13 C vs latitude  13 C (North, <5°S)7°-13°S

11. Results:  13 C vs latitude The latitudinal variation of δ 13 C values in D. gigas muscles and in zooplankton samples could then be related to the upwelling off Peru and reflect latitudinal changes in productivity

12. Results:  13 C vs size  13 C Other papers; Ruiz-Cooley (2006) Related with food web, carbon differences in δ 13 C occur primarily at primary production, with small increases with increasing trophic level (0.4%; Post 2002) Therefore, hypothesizing that δ 13 C values only reflect changes in habitat, the increasing trend of δ 13 C values with ML would suggest that D. gigas might change of habitat while growing. However, the high variability of δ 13 C by size in our work suggests that D. gigas’ juveniles and adults distribute over a large range of habitats

13. Results:  15 N vs latitude Increasing trend (8‰; 3°S-18°S) Ruiz-Cooley (2006) (6‰; 40°N-0°N) Takai (2000) (6‰; Japan – Perú) If the δ 15 N of animal tissues are used as an indicator of trophic position in marine food webs, variation in δ 15 N values in consumers may not only result from the trophic level at which they feed, but also from changes at the base of the food web

14. Results:  15 N vs latitude Baseline isotopic plankton Dissolved inorganic nitrogen Environmental factor (0 2 )

15. Results:  15 N vs latitude Baseline isotopic plankton Dissolved inorganic nitrogen Environmental factor (0 2 ) North…………………………………………………………………………….South nitrates < Oxygen deficient> Oxygen deficient < denitrification <  15 N > denitrification >  15 N

16. Results: Latitudinal variation of δ 15 N values of zooplankton and D. gigas in Peruvian waters A similar trend in D. gigas and zooplankton suggests that baseline isotopic values are also the main drivers of δ 15 N values in jumbo squid muscles. As the slopes did not differ significantly between zooplankton nd jumbo squid we hypothesize that squids of a same size have a similar trophic position whatever the latitude. At the population level, the very high variability in both δ 13 C and δ 15 N values at a given DSB or latitude illustrates that squids in a given area may come from different places and therefore have different life histories in terms of habitats.

17. Results: Isotopes variability and diet VariableSourcee.d.fFP% explained δ 13 CLat3.76837.780< 2 e-1634.40 Size1.00026.5007.10 e-077.80 DSB8.28312.8307.77 e-1534.43 Model < 2 e-1676.63 δ 15 NLat3.62959.419< 2 e-1645.33 Size 8.7639.9453.46 e-12 28.72 DSB2.8537.3664.19 e-056.15 Model < 2 e-1682.20 Statistical outputs of GAMs based on mantle δ13C and δ15N values of D. gigas

18. Results:  15 N vs size  15 N Increasing trend  Ruiz-Cooley (2006) High variability …..opportunistic foraging strategies High variability suggest that during ontogeny squid of different size can prey over same resources

19. Results:  15 N vs size  15 N Increasing trend  Ruiz-Cooley (2006) High variability …..opportunistic foraging strategies High variability suggest that during ontogeny squid of different size can prey over same resources Number of prey species, stomach analyzed and percentage weight of principals prey groups of D. gigas by mantle length range and latitude

20. Results: Isotopes variability and diet This study confirms that D. gigas is a highly opportunistic species with a wide trophic niche (from euphausiids to cephalopods and fish).

21. Dosidicus gigas Results showed a very high variability in both δ 13 C and δ 15 N values at a given DSB, latitude or size which illustrates that squids captured in a given area may have very different life histories. The similar latitudinal trend of isotope values of zooplankton and D. gigas in the study area suggests that D. gigas is a relatively resident species at the scale of its tissue turnover (i.e., a few weeks). The increasing trend of δ 13 C values with mantle length, and the strong relation between δ 13 C values and distance to shelf break suggest that D. gigas migrate from oceanic to coastal waters, changing its foraging areas between juvenile and adults, although no significant diet shift seems to occur. There is no systematic increase in trophic position with size and that latitude is the main driving factor on δ 15 N values off Peru Summary

22. Thanks