1. Protein delivery: DNA nanostructures and cell-surface targeting Harvard iGEM August 27, 2006

2. The Machine Goal: Future modular drug delivery drug target cell

3. Molecular containers in nature Hard to duplicate artificially http://micro.magnet.fsu.edu/cells/viruses/images/virus.jpg http://www.biology4kids.com/files/art/cell_over1.gif

4. DNA Nanostructures Overview DNA can be used to approximate arbitrary 3D structures WILLIAM M. SHIH, JOEL D. QUISPE & GERALDF. JOYCE Nature 427, 618 ﾐ 621 (2004); http://www.dna.caltech.edu/~pwkr/ William Shih, Harvard Ned Seeman, NYUPaul Rothemund, Caltech http://seemanlab4.chem.nyu.edu/nano-cube.html

5. Motivation: Why DNA? The power of DNA Nanometer scale Covalent modifications possible Inexpensive synthesis Highly programmable/designable

6. Design Details: Scaffolded Oragami M13 viral genome 7308 bases long Add ~180 helper strands in Mg++ buffer Heat to near boiling. (90 C)

7. Design Details: Scaffolded Oragami

8. When the sample reaches room temperature (2hrs later), the origami have folded http://www.dna.caltech.edu/~pwkr/

9. Design Details: Positional Control

10. Design Details Double-ply barrel and lid Lid: 33 nm across, 28 nm long Barrel: 27.5 nm long, 27.6 nm across

11. Exciting EM Images

13. To be continued Can a protein be protected from protease if attached inside the box? Lid attachment Lid removal protein protease

14. Acknowledgements Harvard TFs - Shawn Douglas, Nick Stroustrup, Chris Doucette Harvard advisers - Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal iGEM ambassadors iGEM directors