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Published byLynn Marshall Modified over 9 years ago
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Supplementary Data
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β-actin IL-13Rα2 65 kDa 42 kDa G26-H2 G26-V2 SnB19-pcDNA SnB19-asIL-13Rα2 Figure S1A
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10 12 10 910 10 11 10 12 10 1110 10 12 10 11 10 9 10 1210 10 9 10 1110 Phage clones (pfu/ml) 10 9 Pep-1Pep-2Pep-3M13KE Figure S1B 0.0 0.2 0.4 0.6 0.8 DTT (-) DTT (+) O.D. - A 405 nm
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Concentration (µg/ml) Figure S1C 0.5 1.0 2.5 5 0.5 1 2.5 5 0.5 1 2.5 5 0.5 1 2.5 5 0.5 1 2.5 5 0.0 0.2 0.4 0.6 0.8 IL-13R 2-Fc IgG-Fc BSA Pep-1-L Pep-1-DSC Pep-1-L (C/S) Control Pep-1-L Control Pep-1-DSC O.D. - A 405 nm
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1 µg/ml5 µg/ml U-251 MG Pep-1-DSC Pep-1-L Figure S2 A
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Pep-1-L B T98G cells Pep-1-DSC Figure S2 1 µg/ml5 µg/ml
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Scrambled Pep-1-L U-251 MG cells C No peptide 1 µg/ml Figure S2 5 µg/ml
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Figure S2 D
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Figure S3A Strep- Cys5.5 Strep- Cys5.5 Strep- Cys5.5 30 min2 hr8 hr 24 hr72 hr Pep-1-DSC Ctrl. Pep-1-DSC SalinePep-1-DSC Ctrl. Pep-1-DSC SalinePep-1-DSC Ctrl. Pep-1-DSC Saline 48 hr Pep-1-DSC Ctrl. 0 2 4 6 8 p = 0.0148 * Total NIR Radiant efficiency (x 10 ) 10
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2 24 48 120 144 288 Luciferin Figure S3B Pep-1-DSC Ctrl. Strep- Cy5.5 Saline hr
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Figure S4 A B i.ii.iii. i.ii.iii.
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