1. GC-MS based metabolite profiling for flavor characterization of Brassica crops grown with different fertilizer application Tomohiko Fukuda a,d,*, Keiki Okazaki b, Ayano Watanabe e, Takuro Shinano b,c,d, Norikuni Oka b,d a Agricultural Research Institute, HOKUREN Federation of Agricultural Cooperatives, 375 Kita-6, Higashi-7, Higashi-ku, Sapporo 060-0906, Japan b National Agricultural and Food Research Organization/Hokkaido Agricultural Research Center, Hitsujigaoka-1, Toyohira-ku, Sapporo, 062-8555, Japan c National Agricultural and Food Research Organization/Tohoku Agricultural Research Center for Fukushima Region, 50 Harajukuminami, Arai, Fukushima, 960-2156, Japan d Graduate School of Agriculture, Hokkaido University, Kita-21, Nishi-11, Kita-ku, Sapporo, 001-0021, Japan e Food and Agricultural Materials Inspection Center, Shintoshin 2-1, Chuo-ku, Saitama, 330-9731, Japan *Corresponding Author: (T. F.) Address: 375 Kita-6, Higashi-7, Higashi-ku, Sapporo, Hokkaido 060-0906, Japan. E-mail: fukuda-tomohiko@hokuren.jp Supplementary Figures

2. Fig. S1A Supplemental Fig. 1 PCA loading plots based on GC-MS metabolite profiling. (A) Determined by 78 hydrophilic metabolites. The numbers 1–78 correspond to the metabolites in Supplementary Table 2. (B) Determined by 35 volatiles. The numbers 1–35 correspond to the volatiles in Supplementary Table 3. Amino acids Organic acids Sugars Sugar alcohols

3. Fig. S1B Isothiocyanates Other N or S volatiles Terpenoids Aldehydes