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S1R1S1R2S2R1S2R2S1R4 S2R3 S2R4 S1R3 S1R1S1R2S2R1S2R2S1R1S1R2S2R1S2R2 S1R3S1R4S2R3S2R4S1R3S1R4S2R3S2R4 PT SET PT SET PT SET PT SET PT SET PT SET PT SETPT.

Published byHillary Edwards Modified over 9 years ago

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Presentation on theme: "S1R1S1R2S2R1S2R2S1R4 S2R3 S2R4 S1R3 S1R1S1R2S2R1S2R2S1R1S1R2S2R1S2R2 S1R3S1R4S2R3S2R4S1R3S1R4S2R3S2R4 PT SET PT SET PT SET PT SET PT SET PT SET PT SETPT."— Presentation transcript:

1 S1R1S1R2S2R1S2R2S1R4 S2R3 S2R4 S1R3 S1R1S1R2S2R1S2R2S1R1S1R2S2R1S2R2 S1R3S1R4S2R3S2R4S1R3S1R4S2R3S2R4 PT SET PT SET PT SET PT SET PT SET PT SET PT SETPT SET PT- porcine trypsin SET – Streptomyces erythraeus trypsin S1R1S1R2S2R1S2R2S1R1S1R2S2R1S2R2 S1R3S1R4S2R3S2R4S1R3S1R4S2R3S2R4 Protein extraction with B-PER Gel electrophoresis Protein digestion in gel LC-MS/MS analysis – Qstar Elite Database search – Mascot and Xtandem! Protein identification validation and quantitation - Scaffold Optimal biocathodes Suboptimal biocathodes

2 Proteins: 90% protein probability 95% peptide probability At least 1 peptide 579 proteins

3 Peptides: 90% protein probability 95% peptide probability At least 1 peptide

4 Spectra: 90% protein probability 95% peptide probability At least 1 peptide

5 Proteins: 90% protein probability 70% peptide probability At least 2 peptides 286 proteins

6 Peptides: 90% protein probability 70% peptide probability At least 2 peptides

7 Spectra: 90% protein probability 70% peptide probability At least 2 peptides

8 Proteins: 90% protein probability 70% peptide probability At least 2 peptides 286 proteins

9 peptides: 90% protein probability 70% peptide probability At least 2 peptides

10 spectra: 90% protein probability 70% peptide probability At least 2 peptides

11 CategoryBio SampleMS/MS Sample#Prot#IDs#Spec%IDs PTS1R1 PTmascot_daemon_merge (S1R1_gel2_opt_F003330)4320029890.06691201 PTS1R2 PTmascot_daemon_merge (S1R2_gel2_opt_F003339)11067648150.1403946 PTS2R1 PTmascot_daemon_merge (S2R1_gel2_opt_F003342)6426248910.05356778 PTS1R3 PTmascot_daemon_merge (S1R3_gel2_subopt_F003341)5235346160.07647314 PTS1R4 PTmascot_daemon_merge (S1R4_gel2_subopt_F003340)15076062300.12199037 PTS2R3 PTmascot_daemon_merge (S2R3_gel2_subopt_F003343)8541251090.08064201 PTS2R4 PTmascot_daemon_merge (S2R4_gel2_subopt_F003344)4718248570.03747169 PTS2R2 PTmascot_daemon_merge (S2R2_gel2_opt_F003345)6422239330.05644546 SETS1R1 SETmascot_daemon_merge (F003578_S1R1_Oct_9bands)233103334180.30222353 SETS1R3 SETmascot_daemon_merge (F003577_S1R3_Oct_9bands)10637336420.102416255 SETS1R2 SETmascot_daemon_merge (F003582_S1R2_Oct_9bands)19072543130.16809645 SETS1R4 SETmascot_daemon_merge (F003581_S1R4_Oct_9bands)243125070840.17645398 SETS2R1 SETmascot_daemon_merge (F003583_S2R1_Oct_9bands)19054667080.08139535 SETS2R3 SETmascot_daemon_merge (F003584_S2R3_Oct9bands)22575769120.109519675 SETS2R2 SETmascot_daemon_merge (F003586_S2R2_Oct_9bands)20166848120.13881962 SETS2R4 SETmascot_daemon_merge (F003587_S2R4_Oct_9bands)18455947970.11653116

12 Proteins: 90% protein probability 95% peptide probability At least 1 peptide 579 proteins

13 peptides: 90% protein probability 95% peptide probability At least 1 peptide

14 spectra: 90% protein probability 95% peptide probability At least 1 peptide

15 CategoryBio SampleMS/MS Sample#Prot#IDs#Spec%IDs PTS1R1 PTmascot_daemon_merge (S1R1_gel2_opt_F003330)6324329890.08129809 PTS1R2 PTmascot_daemon_merge (S1R2_gel2_opt_F003339)14860248150.12502596 PTS2R1 PTmascot_daemon_merge (S2R1_gel2_optF003342)8727348910.055816807 PTS1R3 PTmascot_daemon_merge (S1R3_gel2_subopt_F003341)7536246160.078422874 PTS1R4 PTmascot_daemon_merge (S1R4_gel2_subopt_F003340)21773862300.11845907 PTS2R3 PTmascot_daemon_merge (S2R3_gel2_subopt_F003343)11142951090.08396947 PTS2R4 PTmascot_daemon_merge (S2R4_gel2_subopt_F003344)7121848570.044883672 PTS2R2 PTmascot_daemon_merge (S2R2_gel2_opt_F003345)8824139330.06127638 SETS1R1 SETmascot_daemon_merge (F003578_S1R1_Oct_9bands)347102834180.3007607 SETS1R3 SETmascot_daemon_merge (F003577_S1R3_Oct_9bands)12137636420.103239976 SETS1R2 SETmascot_daemon_merge (F003582_S1R2_Oct_9bands)24669443130.16090888 SETS1R4 SETmascot_daemon_merge (F003581_S1R4_Oct_9bands)337118070840.16657256 SETS2R1 SETmascot_daemon_merge (F003583_S2R1_Oct_9bands)22648567080.07230173 SETS2R3 SETmascot_daemon_merge (F003584_S2R3_Oct9bands)30872469120.10474537 SETS2R2 SETmascot_daemon_merge (F003586_S2R2_Oct_9bands)27966948120.13902743 SETS2R4 SETmascot_daemon_merge (F003587_S2R4_Oct_9bands)22049347970.10277256

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