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Lecture-7 Genome editing CRISPR
Huseyin Tombuloglu, Phd GBE423 Genomics & Proteomics
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Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins CRISPR/Cas9 System
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What is it? derived from a natural process found in bacteria to protect themselves from pathogens targets genes for editing and regulating comparable to Photoshop Horizon Licenses Harvard University Gene-Editing Technology. (2013). Drug Discovery & Development.
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To initiate gene modification,
sgRNA (single guide RNA) Cas9 complex Cas9 nuclease
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Protospacer Adjacent Motif (PAM)
Target Sequence Gene of Interest
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Non-Homologous End Joining (NHEJ) DNA repair pathway
Stop Codon
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Summation: crispr/Cas system
sgRNA/Cas9 complex binds to gene Cas breaks gene (double strand breaks) Induced mutation in gene sequence altered gene sequence dysfunctional CAD gene
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