Presentation is loading. Please wait.

Presentation is loading. Please wait.

JP Stevens High School December Meeting. Summer Clones…

Published byDana Williamson Modified over 9 years ago

Similar presentations

Presentation on theme: "JP Stevens High School December Meeting. Summer Clones…"— Presentation transcript:

1 JP Stevens High School December Meeting

2 Summer Clones…

3 MUCPUCPUCP Plasmid Digest & PCR Clones 9SP17-19.09 370 bp130 bp550 bp 9SP17.099SP18.099SP19.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp 100bp λ

4 MUCPUCPUCP Plasmid Digest & PCR Clones 9SP20-22.09 180 bp130 bp230 bp 9SP20.099SP21.099SP22.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp 100bp

5 MUCPUCPUCP Plasmid Digest & PCR Clones 9SP23-25.09 130 bp450 bp575 bp 9SP23.099SP24.099SP25.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp 100bp

6 MUCPUCPUCP Plasmid Digest & PCR Clones 9SP26-28.09 240 bp260 bp400 bp 9SP26.099SP27.099SP28.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp

7 MUCPUCPUCP Plasmid Digest & PCR Clones 9SP29-31.09 100 bp** 400 bp **800 bp 9SP29.099SP30.099SP31.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp

8 MUCPUCP Plasmid Digest & PCR Clones 9SP32,37.09 1050 bp90 bp 9SP32.099SP37.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp 100bp Is that a band?

9 MUCPUCPUCP Plasmid Digest & PCR Clones 9SP38-40.09 80 bp800 bp70 bp 9SP38.099SP39.099SP40.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 5000bp 3000bp 4000bp 12000bp 100bp

10 MUCPUCPUCP Plasmid Digest & PCR Clones 9AR15-17.09 1100 bp300 bp400 bp 9AR15.099AR16.099AR17.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp

11 MUCPUCPUCP Plasmid Digest & PCR Clones 9AR18-20.09 100 bp150 bp100 bp 9AR18.099AR19.099AR20.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp

12 MUCPUCPUCP Plasmid Digest & PCR Clones 9AR21-23.09 130 bp110 bp120 bp 9AR21.099AR22.099AR23.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp

13 MUCPUCPUCP Plasmid Digest & PCR Clones 9AR25-27.09 200 bp750 bp220 bp 9AR25.099AR26.099AR27.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp

14 Colony PCR…

15 M Colony PCR Clones 9SA1-3.09, 9AH1-3.09, 9AN1-3.09 170 bp230 bp220 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 800 bp760 bp480 bp230 bp100 bp110 bp SA1SA2SA3AH1AH2AH3AN1AN2AN3

16 M Colony PCR Clones 9AR41-43.09, 9JW1-3.09, 9PS1-3.09 200 bp30 bp180 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 170 bp30 bp 480 bp200 bp290 bp AR41AR42AR43JW1JW2JW3PS1PS2PS3

17 M Colony PCR Clones 9JW4-9.09, 9PS4-6.09 80 bp220 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 220 bp130 bp70 bp230 bp330 bp530 bp JW4JW5JW6JW7 JW9PS4PS5PS6

18 M Colony PCR Clones JW10-18.09 230 bp 220 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 220 bp30 bp220 bp 240 bp JW10JW11JW12JW13JW14JW15JW16JW17JW18

19 M Colony PCR Clones 9SA7-9.09, 9JW19-24.09 230 bp220 bp180 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 180 bp 280 bp430 bp230 bp260 bp SA7SA8SA9JW19JW20JW21JW22JW23JW24

20 M Colony PCR Clones 9SP41-43.09, 9EJ1-3.09, 9AN4-6.09 100 bp70 bp80 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 80 bp680 bp530 bp180 bp190 bp240 bp SP41SP42SP43EJ1EJ2EJ3AN4AN5AN6

21 M Colony PCR Clones 9SA4-609, 9AN7-12.09 230 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 220 bp 230 bp240 bp230 bp SA4SA5SA6AN7AN8AN9AN11AN12AN10

22 M Colony PCR Clones 9JJ17-21.09, 9SP44-49.09 230 bp130 bp180 bp 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp 200 bp80 bp220 bp1050 bp580 bp360 bp380 bp370 bp JJ17JJ18JJ19JJ20JJ21SP44SP45SP46SP47SP48SP49

23 M Colony PCR Clones 9SK1-3.09, 9NN1-3.09 2000bp 1650bp 1000bp 850bp 650bp 500bp 400bp 300bp 200bp 3000bp 4000bp 12000bp 100bp SK1SK2SK3NN1NN2NN3 210 bp280 bp80 bp1230 bp130 bp

Download ppt "JP Stevens High School December Meeting. Summer Clones…"

Similar presentations

CLONING AND EXPRESSION OF NEUTRAL PROTEASE GENE FROM B. STEAROTHERMOPHILUS.

CLONING AND EXPRESSION OF NEUTRAL PROTEASE GENE FROM B. STEAROTHERMOPHILUS.
You start with a biologically relevant protein from a pathogen (Bacterium, virus, parasite…)

You start with a biologically relevant protein from a pathogen (Bacterium, virus, parasite…)
Caffeine Disk Results Spring 2014. Procedure Grow clone from glycerol stock – Amp or amp + chlor – 4 mM caffeine Measure A590 and standardize to 0.1 or.

Caffeine Disk Results Spring Procedure Grow clone from glycerol stock – Amp or amp + chlor – 4 mM caffeine Measure A590 and standardize to 0.1 or.
Verify recombination by electrophoresis. Digest of rfp gene. Transform bacteria with recombinant plasmid. Recombination (ligation) of plasmid and rfp gene.

Verify recombination by electrophoresis. Digest of rfp gene. Transform bacteria with recombinant plasmid. Recombination (ligation) of plasmid and rfp gene.
Cloning Worksheet Winter 2011 Producing a Standard Curve to Determine DNA Fragment Sizes.

Cloning Worksheet Winter 2011 Producing a Standard Curve to Determine DNA Fragment Sizes.
Polymerase Chain Reaction - PCR The photocopier of molecular biology.

Polymerase Chain Reaction - PCR The photocopier of molecular biology.
Understanding the mechanism of action of the Plasmodium falciparum lariat debranching enzyme BME230 Computational Genomics Maria Daleke 03-16-05.

Understanding the mechanism of action of the Plasmodium falciparum lariat debranching enzyme BME230 Computational Genomics Maria Daleke
DNA Science Day 2 Extracting, Ligating and Transforming Physical Biology Bootcamp October 2006 Caltech.

DNA Science Day 2 Extracting, Ligating and Transforming Physical Biology Bootcamp October 2006 Caltech.
DNA Science Day 2 Extracting, Ligating and Transforming APh162 Winter 2005 Caltech.

DNA Science Day 2 Extracting, Ligating and Transforming APh162 Winter 2005 Caltech.
Gel Scramble Lecture 1 William Grisham, Ph.D. Copyright 2014.

Gel Scramble Lecture 1 William Grisham, Ph.D. Copyright 2014.
Epigenetics Lab December 1, 2008 Goals of today’s lab: 1.Understand the basic molecular techniques used in the lab to study epigenetic silencing in cancer.

Epigenetics Lab December 1, 2008 Goals of today’s lab: 1.Understand the basic molecular techniques used in the lab to study epigenetic silencing in cancer.
Gateway cloning system

Gateway cloning system
94VG 175 300 Insert size M 94PCRG##.13 450 210 280 80 800 13 1 1 11 1 1 PCR gel template: Fill in the #s and Xs with the appropriate information 650 1000.

94VG Insert size M 94PCRG## PCR gel template: Fill in the #s and Xs with the appropriate information
IGEM Bioware 7.12-16.2010. Arsenic PCR - 62% Successful! We're still having trouble with LamB and ArsB. Ligations and Transformations have been unsuccessful.

IGEM Bioware Arsenic PCR - 62% Successful! We're still having trouble with LamB and ArsB. Ligations and Transformations have been unsuccessful.
LET’S PLAY THE REVIEW GAME! (a.k.a how much did you forget this summer?)

LET’S PLAY THE REVIEW GAME! (a.k.a how much did you forget this summer?)
Gel pictures of cloning lab. Section1 Gel1 3 kb 5 kb 1.6 kb M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT.

Gel pictures of cloning lab. Section1 Gel1 3 kb 5 kb 1.6 kb M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT.
Restriction Enzyme Vector Ligase Enzyme Recombinant DNA DNA Construct Digestion ligation.

Restriction Enzyme Vector Ligase Enzyme Recombinant DNA DNA Construct Digestion ligation.
Molecular Cloning.

Molecular Cloning.
Today House Keeping (talks, sequencing) Follow-up on cloning

Today House Keeping (talks, sequencing) Follow-up on cloning
Today House Keeping (talks, sequencing) Follow-up on insert check Sequencing Plant DNA extractions 15 minute PPT Sequencher plus BLAST.

Today House Keeping (talks, sequencing) Follow-up on insert check Sequencing Plant DNA extractions 15 minute PPT Sequencher plus BLAST.

Similar presentations

Ads by Google