1. First Annual Meeting

2. CTSI Project Development Team: Benefits a Young Investigator Kimberly K. Buhman, Ph.D. Assistant Professor of Foods and Nutrition Purdue University

3. The Process of Dietary Fat Absorption TG MG +FFA DG +FFA TG CM Enterocyte Intestinal lumen Plasma CM Via Lymph CM = Chylomicron CLD CLD = Cytoplasmic Lipid Droplet Long Term Goal: to identify novel factors that differentially regulate intestinal triglyceride metabolism and may serve as targets or methods for preventive and therapeutic interventions for obesity, CHD, and diabetes.

4. Coherent Anti-Stokes Raman Scattering Microscopy (CARS) Nonlinear Vibrational Imaging Technique with Sub-micron Resolution Advantages Chemical specificity Image LIPIDS in live cells and tissues without labeling - Strong signal from molecules enriched in C-H bonds Can combine with other imaging techniques Ji-Xin Cheng, Sunney Xie, J. Phys. Chem.(feature article), 2004, 108, 827. Ji-Xin Cheng, Applied Spectroscopy (focal point article), 2007, 61:197A. DC 50 µm 20 µm

5. 50 µm 5 µm 50 µm 5 µm Lipid accumulation in mice fed a chronic high fat diet Cytoplasmic Lipid Droplets Observed in Enterocytes of Mice Fed a High Fat Diet LOW FAT HIGH FAT

6. Idea  Funded Project – Drafted specific aims page for grant and identified a potential funding mechanism – Discussed with Project Development Team (PDT) mentor Meeting set up by PDT mentor – Presented my idea and aims to six, senior faculty members Significant, successful grant writing experience – Modified specific aims based on feedback Focus Sell Resources – Wrote grant – Submitted – FUNDED

7. American Heart Association Scientist Development Grant, National Center, 4 year award Title: Regulation of Intestinal Triglyceride Metabolism: Molecular Mechanisms Grant Objective: to identify factors affecting triglyceride synthesis and catabolism in enterocytes capable of regulating storage and secretion of triglyceride and with ultimate potential for regulating blood triglyceride concentrations. Translation: Identification of targets or methods for preventive and therapeutic interventions for obesity, CHD, and diabetes.