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The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,

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Presentation on theme: "The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,"— Presentation transcript:

1 The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD, C. M. AGRAWAL, J. D. MABREY, V. L. SYLVIA, C. H. LOHMANN, and B. D. BOYAN J Bone Joint Surg Am Volume 81(4):452-61 April 1, 1999 ©1999 by The Journal of Bone and Joint Surgery, Inc.

2 Figs. 1-A and 1-B: Scanning electron micrographs of ultra-high molecular weight polyethylene particles isolated from tissue obtained at the revision procedures (original magnification x 1000 and bar = 10.0 micrometers for Fig. 1-A; original magnification x... D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

3 Figs. 1-A and 1-B: Scanning electron micrographs of ultra-high molecular weight polyethylene particles isolated from tissue obtained at the revision procedures (original magnification x 1000 and bar = 10.0 micrometers for Fig. 1-A; original magnification x... D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

4 Fig. 2 Distribution of the sizes, expressed as equivalent circle diameters in micrometers, of the particles of wear debris isolated from tissue obtained at the revision procedures. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

5 Fig. 3 Effect of ultra-high molecular weight polyethylene particles on the number of MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

6 Fig. 4 Comparison of the effects of ultra-high molecular weight polyethylene particles from granulomatous tissue and the insoluble residue from control tissue on the number of cells (A) and the alkaline-phosphatase-specific activity (B). D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

7 Fig. 5 Effect of ultra-high molecular weight polyethylene particles on [3H]-thymidine incorporation by MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

8 Fig. 6 Effect of ultra-high molecular weight polyethylene particles on alkaline-phosphatase- specific activity of MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

9 Fig. 7 Effect of ultra-high molecular weight polyethylene particles on osteocalcin production by MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

10 Fig. 8 Effect of ultra-high molecular weight polyethylene particles on percent collagen production by MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

11 Fig. 9 Effect of ultra-high molecular weight polyethylene particles on [35S]-sulfate incorporation by MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

12 Fig. 10 Effect of ultra-high molecular weight polyethylene particles on prostaglandin-E2 production by MG63 cells. D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61 ©1999 by The Journal of Bone and Joint Surgery, Inc.

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