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کشت نمونه های مختلف و آنتی بیوگرام

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Presentation on theme: "کشت نمونه های مختلف و آنتی بیوگرام"— Presentation transcript:

1 کشت نمونه های مختلف و آنتی بیوگرام

2 Interpreting Microbiology Reports
The first step in performing cultures is to do a Gram stain of the collected sample (not for blood/urine - that is cultured first, then stained).    Gram staining results few WBCs, no organisms seen. many WBCs, many Gram positive cocci in clusters", or something like that

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7 Interpreting Microbiology Reports
Step 2: Identification: Chemical tests to identify bacteria Next the bacteria are cultured and put through biochemical tests to determine their identification.  This takes a while longer than the Gram stain

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9 Interpreting Microbiology Reports
Step 3: Susceptibilities :Finally the bacteria are tested for their susceptibility to various antibiotics.  Once this data is known, we can transition from empiric to definitive therapy if the team has decided that an infection is indeed present.   The lab sometimes tests bacteria against many different antibiotics that we would never actually use - ignore them.

10 Urine culture

11 Urine culture

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13 Different culture methods

14 Streak culture

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16 Female genital system infections

17 Vaginal secretion culture

18 Bacterial vaginosis

19 Clue cell

20 Blood culture

21 Blood culture

22 Tissue culture

23 CSF culture

24 catheter

25 Susceptibility testing (disk diffusion)
Muller Hinton agar Bacterial inoculums concentration( %5 Mc Farland) Antibiotic disks Pens swap

26 Disk diffusion

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31 Quality control of susceptibility testing (Kirby-Bauer )
Antibiotic Disk potency (by control organisms) Mueller –Hinton Agar depth (4 mm) pH ( ) Instrument failure Inoculums concentration (0.5 Mac farland) Temperature (35-37 C) Moisture

32 MIC determination

33 Microdilution

34 Agar dilution

35 E test

36 Gradiant


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