1. Genetic Engineering

2. Clip http://www.bbc.co.uk/programmes/b00plcl4# clips

3. Steps Isolating the gene of interest Building a cloning construct [Gene targeting] Transformation Selection

4. Step 1: Culturing the gene of interest Animation Ecoli Heat shock

5. Eukaryotic vs. Plasmid DNA

6. Restriction Enzymes Endonucleases Creating “sticky ends” Like puzzle pieces

9. Cloning Vector Restriction enzyme sites as part of a multiple cloning site Resistance marker or antibiotic resistance marker Promoter

10. Cloning Methods Plasmid Bacteriophage

11. Bioethics Concerns The Future of Food Monsanto Patents

12. DIY Bio and Biobricks

13. Agrobacterium tumefaciens Agrobacterium Gene gun Horizontal gene transfer

14. Three Vehicles E. coli DNA construction chassis Agrobacterium tumefaciens DNA transfer chassis Arabidopsis thaliana

15. Applications in Research: Fluorescence Tracking experiments Expression studies

17. Brainbow

18. Knock-out and Knock-in Libraries

19. molecular gastronomy the sweet science

20. brazzein protein is 2000X sweeter than sugar

21. miraculin is a flavor inverter

22. Golden Rice

23. Vitamin A deficiency http://goldenrice.org/Content3- Why/why1_vad.html http://goldenrice.org/Content3- Why/why1_vad.html http://ucbiotech.org/resources/presentations /new_talks/shakespear/shakespeare2.html http://ucbiotech.org/resources/presentations /new_talks/shakespear/shakespeare2.html http://www.scidev.net/en/opinions/golden- gm-rice-shines-again.html

24. Genetically Engineered Viruses http://web.mit.edu/newsoffice/2009/virus- battery-0402.html http://web.mit.edu/newsoffice/2009/virus- battery-0402.html

25. RNAi? PCR and extracting genes Lac operon