1. Biology – The Continuity of Life Objective 3 & 4 Objective 3 & 4 –Describe the various ways genetic engineering can change the organisms around us. –Demonstrate how DNA can be manipulated and used by science.

2. Restriction Enzymes What do they do? What do they do? –They cut DNA in one specific spot Where found…? Where found…? –Found in bacteria What is their natural role…? What is their natural role…? –Bacteria use them as defense, to cut out virus DNA that has infected them

3. Restriction Enzymes 2 Examples & their sites… 2 Examples & their sites… –EcoRI –HinDIII …G GATCC… …CCTAG G…

4. Restriction Enzymes What is a sticky end? What is a sticky end? –Some enzymes do not cut straight across DNA –Some leave an “overhang” –Can be used to connect 2 DNA strands What is a RFLP? What is a RFLP? –The small pieces left when a restriction enzyme cuts DNA into pieces