1. Working with rDNA Safety considerations

2. rDNA constructed outside of a living cell from more than one source capable of replicating in a living cell NIH guidelines rDNA encoding insulin http://images-mediawiki- sites.thefullwiki.org/05/4/5/3/5309811984703583.png

3. Risk Assessment: Organism or Vector Consider: host organism –routes of human infection survival outside of lab transfer of genetic material http://upload.wikimedia.org/ wikipedia/commons/3/32/Es cherichiaColi_NIAID.jpg http://upload.wikimedia.org/wiki pedia/commons/4/46/Baculoviru s.jpg http://upload.wikimedia.o rg/wikipedia/commons/a/ a0/Hela_Cells_Image_3 709-PH.jpg http://upload.wikimedia.org/wik ipedia/commons/6/6f/Arabidop sis_thaliana.jpg

4. Risk Assessment: Nature of DNA Sequence Consider: level of product expression biological stability of product coding for production of toxins coding for antibiotic resistance coding for allergens coding for properties that could make host more infectious http://www.artes- biotechnology.com/rootgif/images/diagramm02.jpg