1. Culture media Definition :- Routine culture media
1-Synthetic media :
2-Routine culture media:
Routine culture media
Common ingredients :-
Dehydrated powders
Bacteriological quality

2. Meat extract :- [Lab. Lemco]
Peptone :-
Nitrogen amino acids
Animal & plant proteins
[lean meat - soya bean]
Proteolytic enzymes : Trypsin
Meat extract :- [Lab. Lemco]
Carbon Vitamins Mineral salt
Evaporation of lean meat
Mineral salts :-
Sulphates Phosphates Sod.chloride
Enzymes activity
Isotonic
Water :-

3. Complex inert long chain polysaccharide
Agar :-
Complex inert long chain polysaccharide
Sea weeds
New Zealand Japan
Melting temp. : 90⁰c ⁰c
Solidifying temp. : 39 ⁰c ⁰c
Concentration in media : % [solid]
[semi solid]

4. Three forms according to the consistency :- 1-Solid :-
Agar up to 2%
Plates or bottles
Isolation from specimens
2-Semi – solid :-
Agar < 1% [ %]
Bottles & tubes
Motility
Some biochemical reactions
Preservation
Transport media
3- Fluid / Broth :-
Blood culture bottles
Enrichment media
[ Carbohydrates fermentation ]

5. Types of routine culture media :-
1- Basic or simple media :
Definition :
Nutrient agar nutrient broth
Non-fastidious organisms
staphylococci E.coli

6. Blood agar Chocolate blood agar
2- Enriched media :
Definition :
Blood agar Chocolate blood agar
Fastidious organisms
Streptococcus pyogenes Niesseria

7. Fermentaion : indicator media
3- Differential media :
Definition :
Fermentaion : indicator media
MacConkey agar :
lactose & non-lactose fermenters
Haemolysis :
Blood agar
α- and β hamolysis

8. 4- Selective media : Definition : Dyes : 1) Adding substances
2) Changing the PH
Dyes :
Crystal violet blood agar [S.pyogenes]
Malachite green L.J [Mycobacteria]
Bile salts :
Sodium deoxycholate [DCA]
Salmonella & Shigella

9. Mineral salts :
Sod.chloride [6.5%]
Mannitol salt agar
staphlococci
Chemicals :
Potassium tellurite
Tellurite blood agar
Corynebacteria

10. Antimicrobial agents : [Antibiotics]
Neomycin blood agar [Clostridia]
Thayer-Martin medium

11. Alkaline peptone water : Vibrios
Changing the PH :-
Alkaline peptone water : Vibrios
Sabouraud medium : Fungi

12. 5- Enrichment media :- Definition : Selenite F broth : Salmonella

13. 6- Specialized media :- Definition : Identification media
Antimicrobial Susceptibility Testing media

14. 7- Transport media :- Definition : Preservation
Buffers - Glycerol - Charcoal - Sod.thioglycolate
Staurt - Amies - Cary - Blair

15. Preparation of media 1- Weighing & dissolving : Precautions
Hygroscopic
Dist. or diionized water
Capacity of the container
String or rotating

16. PH testing :- PH meter Sterilization :- Autoclave
Narrow range PH paper
Sterilization :-
Autoclave
Steaming
Filetration

17. Addition of heat sensitive ingredients :-
Waterbath 45⁰c - 50⁰c
Dispensing :-
Petri - dishes containers
Quality control :-
Control strains
Storage :-
Refrigerator 2⁰c - 8⁰c