1. DNA Scissors James C Mitchell and Bernard Yruke Bell Laboratories, Lucent Technologies Lee Ji Youn

2. Introduction Designed strands of DNA Nanomachine ( a pair of scissors) strands of DNA closing strand (TY) removal strand (RY) strand displacement by branch migration closing of the jaws opening of the jaws angle of 10 degree All by “Self-Assembly” drop of free energy

3. It is possible to obtain precision movements of these machines at a small distances (~ 13nm) away from where the closing force is being applied. closureopening coupled precision movement of a pair of jaws

4. DNA Scissors X version, D version : difference in sequence “C3” spacers containing three CH 2 units :incorporated internally in the strands, short enough to be “hinge-like” rather than “spring-like” handles jaws

5. closing strand removing strand (complement of the closing strand) branch migration Mechanism for Operation

6. FRET Observation FRET “Fluorescence Resonance Energy Transfer” between pairs of 5’-TET and 3’-TAMRA dyes emittingquenching Labeling at QY1F, QY2F Determine the angles subtended by the jaws and by the handles (by measuring the distance) open scissors closed scissors jaws 26.0 15.4 handles 46.6 15.3

7. 30 min

8. Gel Electrophoresis Data 2,4,6,8 : closed band 1,3,5,7 : open band cycle 1 2 3 4 difference by closing or dimer formation  confirm!!

9. Designed Dimer Cannot Form Closed Scissors Compare the behaviour of the dimer standard with a closed scissors solution  the dimer bands migrate slower!

10. Conclusions and Possible Application A scissors-like structure may be devised out of DNA A more practical tool than the tweezers

11. laser at 514.5nm

12. There’s a Specific Closing Strands for Each Pair of Scissors