1. Pg. 367

5. Membrane Protein Movement
Tethered to other
Membrane proteins
Single Protein
Tethered to
cytoskeleton
Pg. 385

8. Cytosol
Nucleus
Mitochondria
Chloroplasts ER
Golgi
Endosome
Lysosome
Transmembrane
Secreted

9. Cytosol
Nucleus
Mitochondria
Chloroplasts ER
Golgi
Endosome
Lysosome
Transmembrane
Secreted

10. The Nucleus

11. Nuclear Structure

12. Nuclear Lamina

13. Nuclear Structure

14. Nuclear Pore Complex

15. Protein Transport to the Nucleus
Nuclear Localization Signal (NLS)
-Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val- N C
Table 15-1 (page 504)

16. NLS is Necessary for Nuclear Import
Protein with
NLS
Nucleus
Nuclear protein with
NLS removed
from protein
Pg. 504

17. NLS is Sufficient for Nuclear Import
Nucleus
Nuclear protein with
NLS removed
from protein
Cytosolic Protein
Without NLS
With NLS
Pg. 504

18. Nuclear Import Pathway

19. Pg. 541

20. Regulated Nuclear Import
NLS “masked”
Allostery!
NLS “exposed”
Pg. 542

21. Cytosol
Nucleus
Mitochondria
Chloroplasts ER
Golgi
Endosome
Lysosome
Transmembrane
Secreted

22. ER Import Sequence Table 15-1 (page 504) Recognition Sequence
N-Met-Met-Ser-Phe-Val-Ser-Leu-Leu-Leu-Val-Gly-Ile-Ile-Phe-Trp-Ala-Thr- C
Hydrolysis of this
peptide bond by
Signal Peptidase enzyme
Table 15-1 (page 504)

24. N

30. Membrane “budding” N C N C N C N C N C

32. Clathrin “triskelion”

33. Clathrin “Cage”

35. Dynamin GTP Hydrolysis

36. Vesicle Fusion with Membrane

37. v-SNARE/t-SNARE Interaction

38. Cytosol Outside of cell Membrane fusion step is regulated Secretory
vesicle
Ca++
Outside of cell

39. Botulism Toxin
Clostridium botulinum
Protease

40. Botulism Toxin
t-SNARE (green)
Botulism Toxin (tan)

41. Botulism Toxin
Botulism Toxin
Protease