1. WORKPACKAGE 2.?: DELIVERABLE 2.?.?: PILLAR 2: Control and intervention strategies along the fork-to-farm chain to ensure beef safety Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens

2. Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens PILLAR 2, WP2.?, D2.?.? Experimental procedure Beef fillets (100 g) Three pathogen bacteria o Listeria monocytogenes (mixture of 6 strains) o Salmonella ser. Typhimurium (mixture of 3 strains) o Escherichia coli 0157:H7 (mixture of 3 strains) Three levels of inoculation o High level (10 7 CFU/g) o Medium level (10 5 CFU/g) o Low level (10 3 CFU/g) Meat mincing machine

3. Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens PILLAR 2, WP2.?, D2.?.? Experimental procedure 1st Beef fillet-Inoculated 7th Beef fillet-Non inoculated 6th Beef fillet - Non inoculated 5th Beef fillet - Non inoculated 4th Beef fillet - Non inoculated 3rd Beef fillet - Non inoculated 2nd Beef fillet - Non inoculated Meat mincing machine 1st – 7th sample - Contaminated

4. Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens PILLAR 2, WP2.?, D2.?.? Conclusions till now… All the non-inoculated samples were contaminated from meat mincing machine, for all inoculum levels and microorganisms tested A 2-log decrease was observed from 1 st inoculated sample till 7 th non-inoculated for the three pathogen bacteria tested In general, L. monocytogenes, Salm. ser. Typhimurium and E. coli may contaminate sequential meat samples through meat mincing machine and consist a high risk in processing industry

5. Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens PILLAR 2, WP2.?, D2.?.? Future work (…to be done the next 5-6 months) Three pathogen bacteria o Listeria monocytogenes (mixture of 6 strains) o Salmonella ser. Typhimurium (mixture of 3 strains) o Escherichia coli 0157:H7 (mixture of 3 strains) Biofilm formation of these pathogens Control of biofilm contamination at non-inoculated beef fillets (using microbiological and molecular techniques)

6. Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens PILLAR 2, WP2.?, D2.?.? Thank you