1. In vivo and in vitro zebrafish models for CNS axonal regeneration after injury
Abdiel Badillo
Jeffery A. Plunkett, Ph.D.

2. What We Know: Mammals Teleost Fishes Regeneration Inhibited
In contrast to mammals, adult zebrafish will regenerate many axons that project from the brainstem
to the spinal cord after lesion that leads to near 100% functional recovery of swimming.
Mammals
Teleost Fishes
Regeneration Inhibited
Regeneration, functional recovery
SCI site
SCI site

3. in-vivo model of SCI using zebrafish
What role do stem/progenitor cell populations play in the regenerative abilities seen in the zebrafish CNS?
Becker et. al., 1997
Brainstem nuclei:
High regenerative capacity
Low regenerative capacity
Wulliman et. al., 1996

4. Rhombencephalic ventricular
What We Know:
Rhombencephalic ventricular
proliferation zone
mouse
zebrafish
Stem Cell Niches

5. Experimental:
Method: Perform SCI and experimentally identify descending tract axons and analyze brainstem tissues for tracer dyes
FR or FE

6. Method: Analyze stem/progenitor markers in the brainstem near back-traced neurons
FR or FE

7. Result analysis and possible ideas: