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Optical microscopy We want to see more details Magnification Resolution Contrast Simple microscope MENA3100, 22/1-07, OBK.

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Presentation on theme: "Optical microscopy We want to see more details Magnification Resolution Contrast Simple microscope MENA3100, 22/1-07, OBK."— Presentation transcript:

1 Optical microscopy We want to see more details Magnification Resolution Contrast Simple microscope MENA3100, 22/1-07, OBK

2 The simple microscope Antony van Leeuwenhoek (1632−1723)

3 The compound microscope ca. 1595 Hans Janssen Robert Hook (1635−1703) Micrographia, 1664

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5 Stereo microscope

6 Compound microscope for transmitted light

7 Inverted compound microscope for reflected light

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9 Refraction Kromatisk abberation

10 Spherical abberation

11 Corrections The objective

12 Numerical aperture (NA) NA = nsin  R = 0,61 /NA Resolution Rayleigh criterion (Raleigh in Brandon and Kaplan)

13 The limiting factor: Diffraction Oil immersion NA = nsin 

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15 Condenser system Eyepice Ocular (okular) More is needed than just the objective

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18 Optical anisotropy Calcite

19 Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

20 Optically active crystals in the microscope

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22 Michel-Levy chart

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24 Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/46-0009_e.pdf


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