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Optical microscopy We want to see more details Magnification Resolution Contrast Simple microscope MENA3100, 22/1-07, OBK
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The simple microscope Antony van Leeuwenhoek (1632−1723)
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The compound microscope ca. 1595 Hans Janssen Robert Hook (1635−1703) Micrographia, 1664
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Stereo microscope
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Compound microscope for transmitted light
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Inverted compound microscope for reflected light
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Refraction Kromatisk abberation
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Spherical abberation
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Corrections The objective
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Numerical aperture (NA) NA = nsin R = 0,61 /NA Resolution Rayleigh criterion (Raleigh in Brandon and Kaplan)
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The limiting factor: Diffraction Oil immersion NA = nsin
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Condenser system Eyepice Ocular (okular) More is needed than just the objective
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Optical anisotropy Calcite
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Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices
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Optically active crystals in the microscope
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Michel-Levy chart
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Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/46-0009_e.pdf
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