1. Varian 920-LC Competitors

2. 2 Varian 920-LC Market Competitors Competitors Shimadzu Waters Agilent Dionex The 920-LC presents a significant step forward for the Varian

3. Varian 920-LC Compared to the Waters Alliance HPLC

4. 4 Waters Alliance Consists of two versions 2695 Separations Module 2795 Separations Module Pump, autosampler, column heater and controller Pump features independent drive motors –Provides pulse-free operation w/o damper –Limited flow rate of 5 ml/min 5 independent sample carousels –Sample capacity limited to 120 HPLC vials

5. 5 Waters Alliance Complete range of detectors 2998 Diode Array Detector –Only 512 photodiodes – inferior resolution prone to interferences –Limited range of 190 to 600 nm with D2 lamp only 2489 Dual wavelength Detector –Limited range of 190 to 700 nm with D2 lamp only 2475 multi-wavelength Detector 2414 RI Detector 432 Conductivity Detector 2420 ELS Detector 2465 Electrochemical Detector

6. 6 Varian 920-LC Compared to Waters Alliance Waters AllianceVarian 920-LC Maximum flowrate5 ml/min10 ml/min Maximum pressure5000 psi @ 3 ml/min6000 psi @ 5 ml/min Autosampler Capacity 120 HPLC vials200 HPLC vials Microplate CapabilityNA3 plates Max Heater Temp60oC65oC Number of DetectorsNoneUp to 2 built-in Diode Array5121024 UV-Vis Range 190 - 700190 - 900 Sample Carryover<0.1%<0.01%

7. Varian 920-LC compared to the Agilent 1200 Series LC

8. 8 Agilent 1200 Quaternary Pump G1354A Dual piston, low pressure quaternary gradient pump Electromechanical check valve Variable piston stroke Pulse damper between primary and secondary piston Upgrade?? of 1100 series Requires degasser module

9. 9 Agilent 1200 Autosamplers Option of G1329A standard Autosampler capable of 100 HPLC vials G1367B Enhanced Autosampler compatible with either 2 microplates or 108 HPLC vials Standard carryover <0.1% but can be improved to <0.01% using needle wash Both have peltier cooled option

10. 10 Agilent 1200 Detectors Wide range of detectors Variable Wavelength Detector Multi-Wavelength Detectors Diode Array Detector Fluorescence Detector RI Detector

11. 11 Sensitivity of 920-LC UV-Vis vs Agilent 1200 Typical Agilent VWD S/N ~ 25 Typical Varian 920-LC UV/Vis S/N > 36

12. 12 Varian 920-LC Compared to Agilent 1200 Agilent 1200Varian 920-LC Pulse DamperYesNot Required Microplate compatibility Not CompatibleUp to 3 96 well plates Autosampler Capacity 100 HPLC vials200 HPLC vials Dual UV-Vis NAYes Carryover<0.1%<0.01% UV-Vis range 190 – 600 D2 only190 – 900 D2 & QI UV detector noise+ 0.75 x 10 -5 Au+ 0.5 x 10 -5 Au

13. 13 Waters Purification Systems

14. 14 Waters Delta 600 LC Analytical to Prep Delta 600 wide flow rate range and 2 way injector valve allows operation at analytical and prep scales Delta 600 LP –LP dual piston quaternary gradient pump 0.01 to 20 ml/min & up to 6000 psi Up to 45 ml/min option Stand alone 2 channel degasser option recommended

15. 15 PrepLC Dedicated Purification Systems PrepLC 150 and 300 –Dedicated preparative LC system quaternary gradient –1 to 150 ml/min Up to 4000 psi –4 to 300 ml/min Up to 2000 psi DeltaPrep 150 and 300 –Same as PrepLC but with solvent recycling capabilities

16. 16 Waters PrepLC Sample LoadColumn IDModelMax Flow Rate (ml/min) mg – 10’s g7.8 – 75 mmDeltaPrep 300300 ml/min  g – g 4.6 – 50 mmDeltaPrep 150150 ml/min  g – 10’s mg 4.6 – 50 mm2545150 ml/min  g – 10’s mg 3.9 – 30 mmDelta 600 EF45 ml/min  g – 10’s mg 3.9 – 19 mm1525 + EF22.5 ml/min  g – 10’s mg 3.9 – 19 mmDelta 60020 ml/min  g - g 3.9 – 7.8 mm152510 ml/min

17. 17 Waters Detectors for Prep LC

18. Questions??