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Microscope & Simple Epithelium
LAB EXERCISE BY DR. ANSARI Wednesday, April 26, 2017 4/26/2017 HISTOLOGY LAB.1.
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OBJECTIVES Parts of a compound microscope. Function of each part.
Types of microscope. Types of tissues Features of simple epithelium Types of simple epithelium Locations of simple epithelium 4/26/2017
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PARTS OF A COMPOUND MICROSCOPE 4/26/2017
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EYE PIECE The part of the microscope that you look through. Eyepieces in most compound microscopes have a lens with a 10x magnification level. Our Advanced microscope has a pointer built in, which appears as a black line across half of the field of view. To move the pointer, just turn the top of the eyepiece. 4/26/2017 The eye piece/ocular
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The first set of objectives are called low power,
The magnification range is 10X; 4X 4/26/2017 The objectives lenses
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The second set of objectives are called “high Power”
OBJECTIVE LENSES ARE NEAR THE SPECIMEN The second set of objectives are called “high Power” They multiply 40X; & 100X. 100X is oil immersion 4/26/2017 THE HIGH POWER
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CONDENSOR/IRIS IT CONVERGES THE LIGHT ON TO THE SPECIMEN 4/26/2017
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STAGE, HANDLE, ADJUSTMENT KNOBS
NON OPTICAL PARTS ARE STAGE, HANDLE, ADJUSTMENT KNOBS SLIDE MOVEMENT KNOBS 4/26/2017
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TYPES OF MICROSCOPES DARK FIELD MICROSCOPE PHASE CONTRAST MICROSCOPE
FLOURESENT MICROSCOPE ELECTRON MICROSCOPE A beam of electrons are used for illumination and it is recorded and viewed, it increases the resolution A light object is seen on a dark background, e.g.. Spirochetes, syphilis bacteria can be seen by dark field microscope. Live unstained microorganism can be seen by Phase contrast microscope. Ultraviolet light is used to illuminate the specimen by fluorescent microscope. 4/26/2017
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These are fundamental tissues of the body
Types of tissues There are four basic tissues of our body. 1. Epithelial tissue, 2. Connective tissue, 3. Muscular tissue, 4. Nervous tissue. 4/26/2017 These are fundamental tissues of the body
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FEATURES OF SIMPLE EPITHELIUM
It is a single layered structure, the cells are arranged side by side. No spaces in between. No extra cellular material. No blood vessels/nerves… FEATURES OF SIMPLE EPITHELIUM 4/26/2017
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TYPES OF SIMPLE EPITHELIUM
1. SIMPLE COLUMNAR 2.SIMPLE SQUAMOUS 3.SIMPLE CUBOIDAL 4. PSEUDOSTRATIFIED CLASSIFICATION OF SIMPLE EPITHELIUM 4/26/2017
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CILIA ARE MOTILE STRUCTURES ARISING FROM LUMINAL SURFACE OF EPITHELIUM
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SIMPLE COLUMNAR EPITHELIUM
CELLS ARE ARRANGED IN ONE ROW. TALL COLUMNAR CELLS ALL RESTING ON BASEMENT MEMBRANE HELPS IN ABSORPTION/SECRETION 4/26/2017 FEATURES
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SIMPLE COLUMNAR EPITHELIUM
EXAMPLES GALL BLADDER STOMACH INTESTINE UTERINE TUBE SIMPLE COLUMNAR EPITHELIUM 4/26/2017
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SIMPLE SQUAMOUS EPITHELIUM
A single row of thin scale like cells resting on a basement membrane. Helps in perfusion/filtration/lining the blood vessels/peritoneum/pleura/pericardium. Examples are seen in lung alveoli, Bowman’s capsule of kidney. 4/26/2017 FEATURES AND EXAMPLES
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SIMPLE SQUAMOUS EPITHELIUM
SEEN IN THE INNER LINING OF THE BLOOD VESSELS 4/26/2017 TUNICA INTIMA/ENDOTHELIUM
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SIMPLE SQUAMOUS EPITHELIUM
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Simple squamous epithelium
4/26/2017 LUNG ALVEOLI
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SIMPLE CUBOIDAL EPITHELIUM
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SIMPLE CUBOIDAL EPITHELIUM
SEEN IN CONVOLUTED TUBULES OF KIDNEY 4/26/2017
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LINING CELLS OF THYROID FOLLICLES
SIMPLE CUBOIDAL EPITHELIUM SECRETION/EXCRETION/ ABSORPTION FUNCTIONS OF SIMPLE CUBOIDAL EPITHELIUM 4/26/2017
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PSEUDOSTRATIFIED CILIATED COLUMNAR EPITHELIUM
RESPIRATORY EPITHELIUM 4/26/2017 TRACHEA
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FEATURES OF REPIRATORY EPITHELIUM
MULTILAYERED CELLS NUCLEI AT VARIOUS LEVELS SURFACE CELLS HAVING CILIA SIMPLE GOBLET CELLS CONTAINING MUCOUS ALL CELLS REST ON BASEMENT MEMBRANE 4/26/2017
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RESPIRATORY EPITHELIUM
CILIA AT THE FREE APICAL SURFACE 4/26/2017 FALSE APPEARANCE OF MULTILAYERD CELLS
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IDENTIFY THE TYPE OF EPITHELIUM ?
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What type of epithelium is this?
Where it can be found? 4/26/2017
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Identify the type of epithelium?
Where are the places it can be found ? 4/26/2017
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WHAT TYPE OF EPITHELIUM IS THIS?
WRITE ANY 2 FEATURES OF THIS TYPE OF EPITHELIUM. 4/26/2017
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