1. HEALTHY FOOD FOR EUROPE AMARANTH Amaranthus cruentus LIPIDS IN SEED OIL

2. NI Sterols Squalene LLL LOO LLO OOP OPP OOO TAG TAG = triacylglycerols O – oleic acid C18:1 L – linoleic acid C18:2 P – palmitic acid C16:0 NI – non identified (probably FFA) Squalene Standard Sterols (isolated fraction) Amaranth seed oil HPLC of Lipid Classes (LLSD detection)

3. Triacyloglycerols Squalene FFA NI FFA – Free Fatty Acids NI – non identified GC of Amaranth seed oil (direct „cold on column injection”) see next slide for TAG identification

4. POP PLP POS POO PLO PLL SOS SOOOOO OOL OLL LLL Amaranth seed oil – triacyloglycerols by GC (direct „cold on column injection”) O – oleic acid C18:1 L – linoleic acid C18:2 P – palmitic acid C16:0 S – stearic acid C18:0

5. C 14:0 C 16:0 C 16:1 C 16:2 C 17:0 C 17:1 C 18:0 C 18:1  9 C18:1  11 C 18:2 C 20:0 C 18:3  3 C 20:1 C 22:0 C 24:0 Fatty acids C14:0 – myristic C16:0 – palmitic C16:1 – oleopalmitic C17:0 – margaric C18:0 – stearic C18:1 – oleic C18:2 – linoleic (9,12 all cis) C20:0 – arachidic C18:3 – linolenic (9,12,15 all cis) C20:1 – eikozenoic C22:0 – behenic C24:0 – lignoceric Fatty Acid Composition (GC) of Amaranth seed oil (pressed)

6. Squalene Hydrocarbons isolated from Amaranth seed oil (HR GC, „cold on colum injection”) see next slide for traces

7. Squalene Hydrocarbons isolated from Amaranth seed oil (including traces) hydrocarbons (traces)

8. Alpha-T trienol Beta-T Amaranth seed oil extracted with Carbon Dioxide (SFE) Alpha-T trienol Beta-T Delta-T trienol Delta-T Amaranth seed oil extracted with hexane Alpha-T Beta-T Gamma-TDelta-T Tocopherol Standards Tocopherols in Amaranth seed oil (HPLC/UV at 295 nm)

9. Squalene FFA TAG Triacylglycerols Pressure 250 bar Pressure 275 bar Supercritical Fluid Extraction of Amaranth seeds at 50 0 C Pressure 320 bar Squalene FFA FFA – Free Fatty Acids