1.  Strawberry DNA Extraction Forensic Science 12/8/14

2. Drill  Do strawberries have DNA? How do you know?  What does the A in DNA stand for? Is this soluble in water?  What would you expect to happen if a substance was NOT soluble in a given solvent?  HW: Finish the Lab Qs, if needed

3. Objectives  IWBAT  Extract DNA from a strawberry.  Explain DNA extraction.  Review DNA information.

4. Agenda  Drill  Lab – Extraction of DNA from a Strawberry  Finish PCR Notes – Take these with you to the lab!!  Finish Qs for DNA Extraction: Strawberry  Closure

5.  Some notes:  Each group gets ONE large or TWO small strawberries. Be careful—the juice may stain.  The buffer and ethanol will be on the teacher’s table.  Use the YELLOW graduated cylinder for the DNA extraction buffer  Use the RED/WHITE marked grad cylinder for the ethanol. For the ethanol, you will pour it into a beaker, and then into the grad cylinder.  When done, throw away the cheesecloth and strawberry goo  Everything else should be rinsed out thoroughly, including the plastic bag  While the slurry (goo) is dripping, we will do the PCR Notes. DNA Extraction: Strawberry

6. Pd. 3 Lab Groups Lab StationGroupPartners 11 Boone, Brett WSmith, Alexander D 2 Ruggles, Kayd KFergusson, Tyler A 23 Taylor, Reilly AReider, Christopher J 4 Bledsoe, Michaih EAddai, Kayla A 35 Freeman, Nicole EMiller, Eric M 6 Merrill, Anastasia DHavrilko, Juliana K 47 Keys, Ena DMajid, Macy 8 Fischer, Keenan MGarner, Nathaniel D 59 Barbour, Logan MFiorello, Kathleen M 10 Reyna, MarleyLovitt, Julian A 611 Eggleston, Katherine SHall, Jordan M 12 Calderwood, Michael SSavage, Najee' K 713 Siddiqi, Ashif MCholak, Maggie J 14 Miguelino, Nicholas EBohan, Reid Glenn, Rose M

7. Pd. 5 Lab Groups Lab StationGroup 11 Sheahin, Kevin ACain, James N 2 Banh, BrandonHiggins, Sean D 23 O'Donnell, MaxQadir, Sheeba A 4 Lowenkron, Kyle SWade, Madeline R 35 Ford, Cody AHinson, Avery J 6 Trevizo, Brynn ESavje, Kelsey N 47 Ton, Mary LSchlemm, Andrew M 8 Watson, Jacob CPalmer, Jessica A 59 Truong, AlyssaPatton, Michael C 10 Eder, William MPoligardo, Jesse P 611 Barnhouse, Jeffrey LHaupt, Alyssa M 12 Blair, Drew PIpanaque, Jahaira D 713 Tray, Isaac AKorotkin, Mitchell S 14 Burkindine, Austin RGreengold, Deborah A Johnson, Jaylen M

8. Pd. 6 Lab Groups Lab StationGroup 11 Nadeem, BenMyers, Nyara N 2Hastings, Austin Cole, Christopher C 23 Conver, Caitlyn RSaunders, Nicholas L 4 Ombolo, Stephan MBrown, Brianna N 35 Tray, Mitchell JKreeger, Mariya L 6 Meyer, Allyson JWilliams, Nicholas R 47 Murphy, Matthew CAbbey, Augustus K 8 Boyer, Gavin KOgden, Michael J 59 Grant, Marisa NAbdou, Nicole 10 Casas, JulianHendrickson-Wise, Jordan J 611 Strawley, John CCrawford, Casey N 12 Bassler, Michael REyes, Katherine D 713 Owusu-Sakyi, AlexiaFurr, Nicholas M 14 Kisak, Natalie AGillespie, Stephanie J Gomes, Aldrin

9. Closure  Strawberries are octoploid. Humans are diploid.  We have 2 sets of chromosomes. How many do you think strawberries have?