1. Hands-On Exercise: Grain Yield after Milling RiceCAPS Gramene Workshop 6/8/06

2. 1. Conduct a trait search for milling yield.

3. The QTL search for “milling yield” came up empty. Don’t give up yet: Search site or Literature Search Ontologies

4. Search the whole database for information on it

8. Determine synonyms Or: the ontologies option for searching

9. Search Ontologies Try a different term. “milling ” Try a different term. “mill ”

10. Search for “mill”

15. Back to CMap view

22. View in browser

24. Zoom in/out to a desired level of resolution Features! Those above your sequence are on the forward strand, ones below are on the reverse strand Select options to customize the view. Click on a feature to open menu options

26. Diversity

28. Lemont Teqing

29. Compare Germplasm in Diversity

30. Intro to Pathways – search by gene

33. A line from a gene to a circle - the gene codes for the protein Two circles linked together - a gene product is a subunit of a protein complex Circle connected to reaction -reaction is catalyzed by enzyme. Protein complexes EC number of catalyzing enzyme Gene

34. Compound (red text). Click for more info Reaction (blue line) Click to go to reaction page

35. Thank You! In this exercise you have simply begun to learn what Gramene can do. For more complete details on all the features available from each module, review the module tutorials available on the web. Use the feedback button at the top of every page to submit private questions to the Gramene team. The appropriate person will get back to you. For public discussion, join the Gramene listserve.