1. Are you curious who has been going through your window seats? Who’s been getting into the cookies before dinner? Then “Ms. Mary’s Fingerprinting cottage” is the place for you!

2. Finger printing is a very intricate job that involves techniques such as “Restriction enzymes” and “Gel electrophoresis. Ms. Mary’s has a science team that does all this just for you!

3. First step is PCR Analysis Polymerase chain reaction (PCR) is used to make millions of exact copies of DNA from a biological sample. DNA amplification with PCR allows DNA analysis on biological samples as small as a few skin cells.

4. The technique of DNA fingerprinting requires that the DNA be cut up into small fragments. Restriction enzymes are used to perform this digestion. The technique of DNA fingerprinting requires that the DNA be cut up into small fragments. Restriction enzymes are used to perform this digestion.

5. Then… In RFLP analysis, the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel electrophoresis. In RFLP analysis, the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel electrophoresis.

6. Restriction enzymes always cut at the same base sequence. Because no two individuals have identical DNA, no two individuals will have the same length fragments. Different people are going to have different numbers of this particular sequence and will therefore have different fragment lengths. In addition, some of them will be at different locations on the chromosome. Restriction enzymes always cut at the same base sequence. Because no two individuals have identical DNA, no two individuals will have the same length fragments. Different people are going to have different numbers of this particular sequence and will therefore have different fragment lengths. In addition, some of them will be at different locations on the chromosome.

7. Electrophoresis is a technique used to separate the DNA fragments according to their size. They are placed on a sheet of gelatin and an electric current is applied to the sheet. DNA is charged and will move in an electric field toward the positive pole. Electrophoresis is a technique used to separate the DNA fragments according to their size. They are placed on a sheet of gelatin and an electric current is applied to the sheet. DNA is charged and will move in an electric field toward the positive pole.

8. The smallest fragments will move the fastest because they are able to move through the pores in the gelatin faster. Bands will be produced on the gelatin where the fragments accumulate. The shortest fragments will accumulate near one end of the gelatin and the longer, slower-moving ones will remain near the other end. The smallest fragments will move the fastest because they are able to move through the pores in the gelatin faster. Bands will be produced on the gelatin where the fragments accumulate. The shortest fragments will accumulate near one end of the gelatin and the longer, slower-moving ones will remain near the other end.

9. WAIT BUT THAT’S NOT ALL!

10. Ms. Mary wants to give you a deal you will never believe. Just for $30.99, you will get your culprits figured out and we will add in a months supply of Strawches. A product of a strawberry and peach. We will show you how strawches are made.

11. First step Southern blotting: routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. Southern blotting: routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

12. Second step The polymerase chain reaction (PCR) is used for DNA sequencing in cloning since it amplifies a single or a few copies of a piece of DNA across several orders of magnitude.

13. Third step Complementary DNA is often used in gene cloning. When scientists transfer a gene from one cell into another cell in order to express the new genetic material as a protein in the recipient cell, Complementary DNA is often used in gene cloning. When scientists transfer a gene from one cell into another cell in order to express the new genetic material as a protein in the recipient cell,

14. Continued.. the cDNA will be added to the recipient, because the DNA for an entire gene may include DNA that does not code for the protein or that interrupts the coding sequence of the protein.Partial sequences of cDNAs are often obtained as expressed sequence tags. the cDNA will be added to the recipient, because the DNA for an entire gene may include DNA that does not code for the protein or that interrupts the coding sequence of the protein.Partial sequences of cDNAs are often obtained as expressed sequence tags.

15. Last step We then put the strawches in a cooling machine that makes it juicy and fresh with a subtle taste of ice. So don’t miss out on this great deal! So don’t miss out on this great deal! Call now!!!!! Call now!!!!!