1. Kefir intake as adjuvant onto glycemic control in diabetic rats
Cristina Stewart Bogsan
Pharmaceutical-Biochemical Technology Department

2. Outline Type I Diabettes Mellitus Kefir; Aim; Protocol;
Oxidative stress;
Results;
Conclusion.

3. Diabetes Mellitus
Source:
Source: Shaw, 2010

5. Source: https://alissajhunt. wordpress

6. Source: http://www. thetribuneregister

7. Source: http://www.123rf.com/photo_11271329_pancreatic-islet-normal-and-type-1-diabetic.html

8. Source: http://liberatehealth

9. Source: https://www. diabeticpick

10. Source: http://www. nytimes

11. Kefir
Kefir is a fermented milk that contains a complex symbiotic mixture of Lactic Acid Bacteria (LAB) and Molds.
The main microorganisms are:
Lactobacillus,
Lactococcus,
Leuconostoc,
Streptococcus,
Kluyveromyces,
Saccharomyces,
Torula.

12. Kefir properties
Kefir is known for providing benefits to human health through its anti-inflammatory, immune-stimulatory and antioxidant properties.

13. AIM
This study aimed at assessing the effects of Kefir on oxidative stress and restoration of NO and in immunemodulation in diabetic animals.

14. STZ - diabetic induction
45 mg/kg iv
Kefir preparation
Skimmed Milk
Fermentation until pH 4.6
Stiring(1min)
Distribution in 50 mL cups
Cooling in ice bath
Storing (4 °C)
20mg/ 100mL Kefir DA Inoculation
Kefir fermented milk
Source:

15. Kefir groups intake 1.8 mL/day by gavage Water to CTL and DM groups
Protocol
STZ
Kefir
5 days
Kefir groups intake 1.8 mL/day by gavage
Water to CTL and DM groups
Water and Chow intake
Diuresis, Weight
Fasting Blood tolerance
Creatinine,Proteinuria, Tbars, NO
24 hs
Euthanasia
Immune Function Tests
8 weeks
CTL
CTLK DM
DMK
Water and Chow intake
Diuresis, Weight
Fasting Blood tolerance
Creatinine,Proteinuria, Tbars, NO

16. Oxidative Stress in DM HYPERGLYCEMIA Increased ROS production
Impaired NO bioavailability
NF-kB Activation
Lipid peroxidation (LPO)
Endothelial dysfunction
Pro-inflamatory biomarkers
Malandialdehyde (MPA)
Impair Immune function
Thiobarbituric acid substances (TBARS)
Punaro et al. 2014; Maciel et al. 2015

17. Metabolic profile
Punaro et al. 2014

18. Metabolic profile
Punaro et al. 2014

19. Oxidative Stress
Punaro et al. 2014

20. Oxidative Stress
Punaro et al. 2014

21. Inflammatory biomarker
Punaro et al. 2014

22. Phagocity Activity
Maciel et al, 2015

23. Peyer’s Patchs
Maciel et al, 2015

24. Oral Glucose Tolerance Test
Punaro et al. 2014

25. Glycemia levels
Punaro et al. 2014

26. Conclusion
The results obtained in this study show that Kefir treatment significantly reduced the progression of STZ-induced hyperglycaemia, oxidative stress and potentialize the immune response modulatation in rats. Kefir may play a role in slowing the metabolic changes that contribute to DM as a non-pharmacological adjuvant improving the immunocompetence to better control of glycaemia, reducing or delaying the onset of complications associated with this disease.

27. This work was supported by:
Acknowledgments
Unifesp
Elisa M.S. Higa
Giovana R. Punaro
Fabiane R. Maciel
Adelson M. Rodrigues
Silvia S.M. Ihara
Sergio R.R. Araujo
USP
Marcelo M. Rogero
Marice N. Oliveira
Talita R.C. Sanches
Lucia C. Andrade
This work was supported by:
Coordenação de Aperfeicoamento de Pessoal de Nivel Superior (CAPES),
Fundação de Apoio a Universidade Federal de São Paulo (FAP-Unifesp),
Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP).

28. Thank you