1. Learning how to use the microscope

2. There are 2 kinds of microscopes: Simple: one lens (magnifying glass) Compound: 2 or more lenses *We use compound microscopes in class!

3. Parts of the Microscope Revolving Nosepiece 1. Revolving Nosepiece

4. Parts of the Microscope 2. Lens/Objective

5. Parts of the Microscope 3. Stage

6. Parts of the Microscope 4. Disc Diaphragm (controls amount of ) (controls amount of light) 1 = lowest setting/ 1 = lowest setting/ 5 = highest setting

7. Parts of the Microscope 5. Light source

8. Parts of the Microscope 6. Base

9. Parts of the Microscope 7. Eyepiece

10. Parts of the Microscope 8. Arm

11. Parts of the Microscope 9. Stage Clips

12. Parts of the Microscope 10. Coarse Focus/ Adjustment (Big Knob)

13. Parts of the Microscope 11. Fine Focus/ Adjustment (Small Knob)

14. Calculating Magnification Multiply lens powers together!

15. EyepieceEyepiece: 10 X by itself Low Power:Low Power: 4X; red color; and a TOTAL magnification of 40 X mainly used for large objects/scanning Medium Power:Medium Power: 10 X, yellow color; TOTAL magnification of 100 X High Power:High Power: 40 X; blue color; and a TOTAL magnification of 400 X; mainly used for smaller objects/details

16. When you are ready to start: Make sure it is on LOW power! The stage should be all the way down (you focus by slowly moving it up!) Check the light setting (should be at 1-2 to start)

17. Other Things to Consider: Carrying the Microscope: Always use 2 hands!

18. Other Things to Consider: Never use the COARSE FOCUS KNOB while on HIGH power.

19. When putting the microscope away (at the end of the day): It should be on LOW power. The cord should be WRAPPED around the base. The stage should be all the way DOWN (do this with the coarse focus knob!) The light switch should be OFF

20. TROUBLESHOOTING What if you can’t get your object into focus? Check that you have one of the lenses clicked into place Check that your disc diaphragm is in place (not IN BETWEEN settings)

21. TROUBLESHOOTING What if you can’t get your object into focus? Clean off your lenses using LENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!) GO BACK TO LOW POWER AND START OVER!

22. Instead of a beam of light, these microscopes use a beam of electrons as their source of illumination! Instead of using glass lenses to focus the light, they use a MAGNETIC field to focus the beam of electrons! Used to see things at much higher level of detail Can magnify images up to 1,000,000 times!

23. There are 2 kinds: SCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACES of objects

24. There are 2 kinds: TRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGH an object.

25. Visit this Website to check out some really cool Electron Microscope Images and then answer the questions on your paper! http://www.pbrc.hawaii.edu/bemf/microangela/