1. Aim: Stomata Lab Please Do Now: 1) What is a stomata?
2) What surrounds stomata?
3) What goes into stomata?
4) What goes out of stomata?
5) When are stomata most likely to be open?

2. Agenda
Do Now
Stomata Lab

3. Lab Instructions Same Lab groups as last week Overview of steps:
1. Paint the nail polish on the leaf and LET IT DRY
2. Press a piece of tape down over the dry nail polish and peel it back
3. Take the tape with the STICKY SIDE UP and put it on a microscope slide
4. focus the scope, locate and count the stomata

4. Focusing the scope
Focus with the low power objective first (big knob), then the fine focus (small knob)
To count the stomata in a “field of view” means to count how many you can see at any point looking in the microscope
You should reposition the slide and refocus it to get a new field of view 3 times for each kind of leaf

5. Things to remember for the lab
To get an impression:
Let the nail polish dry
Peel the tape off gently
Put the tape on the slide sticky side up