1. A organic compound mystery lab
Who stole aaron’s ipod
A organic compound mystery lab

2. Lab objectives
Use chemical indicators to determine what organic compound is in a particular food
Accurately collect and record data using tables
Determine who stole Aaron’s Ipod using the chemical composition of a particular food and comparing it with the evidence food and the lunch eaten by each of Aaron’s co-workers.

3. What organic compounds do you think they contain?
Pretzels
Cheese
Butter
Jelly
Fat-free yogurt
Beans –mashed
Foods to be tested
What organic compounds do you think they contain?

4. Paper bags for the
Lipid Test
Well-plates to hold food for the
Biuret and Iodine Tests
Test tubes and rack for
Benedict’s Test

5. Benedict’s test for Glucose
Benedict’s initial color is blue and will change to orange if glucose is present. These tubes are showing a negative result.

6. Brown bag Lipid Test
Food will be rubbed on bag with excess wiped off and air dried. Food with translucent grease spot is positive result

7. Foods from left to Right are yogurt, pretzels, jelly, butter, cheese and beans

8. Starting the Iodine and Biuret TEst
Examine the two colors that each indicator can be.
Iodine – orange
Biuret - blue

9. Foods being tested using Iodine and Biuret Solution
Foods being tested using Iodine and Biuret Solution. What color shows a starch and protein?

10. Food
Carbohydrate Tests
Protein Test
Lipid Test
Color of Benedict’s
Glucose Present
Color of Iodine
Starch Present
Color of Biuret
Protein Present?
Lipids Present
Pretzel (crumbled into the container)
 blue
 no
Black
Yes
Purple No
Cheese
Orange
Butter
Blue
Jelly
 orange
 yes no
Fat – Free yogurt
Beans (mashed into a paste)
Evidence 1
blue
yes
Evidence 2

11. Nutritional information

12. Determine the organic compounds in the foods that each co-worker ate
Data Analysis
Determine the organic compounds in the foods that each co-worker ate

13. Lunch he or she was eating
Who stole Aaron’s Ipod?
Worker in break room
Lunch he or she was eating
Glucose
Starch
Protein
Lipid
Jose
Bean burrito with cheese  x
Ashley
Fat-Free Yogurt
Bruce
Toast with butter and jelly
Kiara
Pretzel
Possible
Thief
Evidence 1 and 2

14. Results for the Lipid Test

15. Post Lab Questions Think about Questions 5 and 6 on lab.
Complete the table in the lab that summarizes the evidence and interpretation of the evidence.
What happened to Aarons Ipod? Explain

16. Energy in Food Energy in food is measured in Calories.
Carbohydrates provide 4 Calories per gram
Proteins provide 4 Calories per gram
Lipids provide 9 Calories per gram
Calculating food Calories
Read the food label.
Determine the number of grams per serving for a given organic compound.
Multiple the number of grams by the number of calories/g for the given food.
Let’s practice…

17. 1. What is the total Calories for one serving?
2. What is the number of Calories from Fat?
3. How many Calories come from Protein?
4. How many Calories come from Carbohydrates1?’
5. Is Sodium an organic compound?
6. Name the two types of fats.