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Size Exclusion Chromatography *
Purification of nucleic acids and proteins Desalting Buffer exchange Dye terminator removal * = Gel Filtration
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Size Exclusion Chromatography
Principle: 1. larger molecules pass the matrix faster 2. small molecules, which can enter the pores of the matrix particles, migrate slowly
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Size Exclusion Chromatography
column preparation 2. column equilibration 3. sample application Volumes available: 1 ml, 200 µl, 15 µl [96 well plates] 4. elute fractions
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Size Exclusion Chromatography
Excellent performance of DextraSEC Columns Comparison of e.g. the removal of ammonia from oligonucleotides NAP 10 Column (GE Healthcare) DextraSEC NA10 AppliChem Higher purity within a smaller elution volume
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Size Exclusion Chromatography
AppliChem's products available: AppliXchange-G25 M DextraSEC Columns DextraSEC Plates
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Size Exclusion Chromatography
AppliChem's sales arguments: over 90 % recovery high chemical stability high resolution easy-to-use best price – best value for money
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Size Exclusion Chromatography
A Survey of the market: (1) Matrix substances Supplier Matrix Amount Price* AppliChem Dextran-based matrix: AppliXchange G25 M 100 g € 192,60 Sepharose-based matrix Illustra Sephadex G-25 GE Healthcare 100 g € 203,- BioRad Polyacrylamide-based matrix Bio-Gel P-6DG Gel 100 g € 269,- *: acc. to Euro price lists 2009/10
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Size Exclusion Chromatography
A Survey of the market: (2) ready-to-use columns Supplier Name Amount Price* AppliChem DextraSEC NA10 50 columns € 255,- GE Healthcare illustra NAP 10 50 columns € 299,- Econo-Pac 10DG 30 columns € 340,- BioRad *: acc. to Euro price lists 2009/10
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Size Exclusion Chromatography
Literature
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