1. Enzymes PP 35-37, 57

2.  Energy: the ability to do work  Requires energy to start the reaction (activation energy, E a ) Chem Rxns Review

3.  End in –ase (i.e. synthase, polymerase, helicase)  Reduces the activation energy (E a )  Protein catalyst

4. Each enzyme specific to a chemical rxn Helps assemble the product OR disassemble the reactants Changes shape when attached to the substrate (reactants) Not used up in the rxn, can be used over and over

5.  Every reaction has reactants that yield products Reactants  Products  Potatoes contain an enzyme called catalase which can break down hydrogen peroxide 2H 2 O 2  2H 2 O + O 2  How do you know it was working?

6. SUMMING UP YOUR OBSERVATIONS  Temp increase activity only to a certain point  Call this optimal temp  Temp changes the shape of the protein (enzyme)

7. SUMMING UP YOUR OBSERVATIONS  Enzymes have a specific pH they can work within  Too low or too high and activity slows or even stops

8.  Concentration  More enzyme  more work and more product  Point of saturation: enzymes can only work so fast even if you add more substrate  Max out on activity