1. KES 2004
Plasmid-DNAgram: Anagram Solving by Molecular Computing Based on GFP-Expressing Plasmid DNA
김수동3 박형주2 이승희2 이은석3 강봉균2, 3 장병탁1, 3
1School of Computer Science & Engineering, SNU
2School of Biological Sciences, SNU
3Interdisciplinary Program in Cognitive Science, SNU

2. Introduction (1) Biosequence exegesis
Charles Lutwidge Dodgson (a.k.a. Lewis Carroll)
Mathematician
His fascination:
Linguistics, Symbolic Logic, Number Theory, Cryptography,
Probability Theory, & Geometry
The author of Alice books
Sequence
Structure
Function
Role
Lexicon
Syntax
Semantics
Fragmatics

3. Introduction (2) Plasmid-DNAgram
FRET (Fluorescent Resonance Energy Transfer)
BFP-GFP pair
CFP-YFP pair
GFP-RFP pair (?)
Cameleon FRET
Cameleon : chimeric protein (CFP-CaM-M13-YFP)
[Ca2+] : CaM-M13

4. Biomolecular FRET for Plasmid-DNAgram

5. Anagram “Rearrange the letters of ‘nor do we’ to make one word.”
‘Herlock Sholmes’ vs. ‘Sherlock Holmes’

6. Plasmid-DNAgram

7. Fluorescent Resonance Energy Transfer
Förster equation

8. Biomolecular FRET

9. Cameleon FRET

10. Cameleon FRET for Plasmid-DNAgram
Sequence
Structure
Function
Role
Lexicon
Syntax
Semantics
Fragmatics
GFP
CFP/YFP
FRET
[Ca2+]

11. Biomolecular FRET for Plasmid DNAgram
HEK293T cell
CFP
FRET
YFP