1. PowerPoint Portfolio By John A Tran 714-913-3531 www.johnatran.com info@johnatran.com

2. About Me Education –Pharmacology Ph.D, 2008 University of California, Irvine –Pharmacological Chemistry B.S., 2002 University of California, San Diego Skills –Published articles in reputable scientific journals on research findings –Presented posters and slide presentations garnering praise and earning prestigious awards –Computer savvy Microsoft Office – especially PowerPoint (animations) EndNote – reference manager Web research – PubMed, Google, etc –A hard worker with the mentality to always get the job done producing quality work

3. Samples of My PowerPoint Work

4. Receptor Theory Basic pharmacological principles –Drug (agonist) binding to a receptor leading to the activation of response in a cell

5. Receptor Theory Stimulus – Agonist binding and receptor activation Transducer – downstream signaling mechanisms Response – result of receptor activation that is measured Cell

6. Characterization of Agonist Activity Estimation of agonist properties from a response plotted on an agonist-response curve

7. Characterization of Agonist Activity EC 50 – concentration of agonist that elicits half-maximal response E max – maximal response

8. CHO-M 2 Cells Emphasizing the first panel (upper left) during a presentation

9. CHO-M 2 cells John A Tran et al (2008) Estimation of Relative Microscopic Affinity Constants of Agonists for the Active State of the Receptor in Functional Studies on M2 and M3 Receptors. Mol Pharmacol. (in press)

10. G Protein Signaling Agonist bound to receptor may induce signaling through one or two G proteins in a cell

11. G1G2

12. M 3 KO and M 2 KO Using a box to highlight the panel that is discussed and its transition

13. M 2 /M 3 KO M 2 KO John A Tran et al (2008) Estimation of Relative Microscopic Affinity Constants of Agonists for the Active State of the Receptor in Functional Studies on M2 and M3 Receptors. Mol Pharmacol. (in press)

14. Cause and Effect – Receptors Cause and Effect –  & R Receptors Arrow Diagram: Shows the inhibition of one of two  receptors    and the consequential increase in contraction Pictorial Diagram: Shows the decrease in response in a cell after the disappearance of the R1 receptor

15. 11 33 (-) (+) Contraction X

16. R1R2

17. Summary A unique way to summarize the presentation

18. Summary The RA i is a relative measure of microscopic affinity constants of the active state of the receptor The RA i is the product of affinity and efficacy of the test agonist relative to a standard agonist The RC analysis can be used in knockout studies to determine the contribution of various receptor subtypes to the response measured

19. The End This is small sampling of what is possible with Microsoft PowerPoint I hope this provides you with an idea of my skill set Any questions? Please contact: John A Tran 714-913-3531 www.johnatran.com info@johnatran.com