1. SEROTYPE DISTRIBUTION OF STREPTOCOCCUS MUTANS IN 3 – 5 YEARS OLD CHILDREN M. Fahlevi Rizal*, B.M. Bachtiar** *Department of Pediatric Dentistry Faculty of Dentistry University of Indonesia **Department of Oral Biology Faculty of Dentistry University of Indonesia

2. Introduction The involvement of Streptococcus mutans in dental caries phatogenesis has been known Little information with regards to the serotype distribution Caries prevalence in Indonesia 63% (SKRT Indonesia Health Dept. 1995) Caries in 3 -5 years old still main problem in Indonesia Covering dental health services in Indonesia : 55%

3. Objective In this study to determine the serotype distribution of S. mutans in children(3-5 years old) using bottle milk in Jakarta.

4. Methods Sample were 34 children between 3- 5 years old who used bottle milk everyday Dental plaque and saliva samples were collected from all subjects Methods

5. Streptococcus mutans was grown on TYS20B Protocol Standard of DNA extraction was done to Streptococcus mutans colony PCR-based serotyping for c, e and f serotype was done following Shibata et al (2003) methods

6. PCR Target of f, e and c serotype Streptococcus mutans f serotype 316 bp e serotype 517 bp c serotype 727 bp M 500 bp 1000 bp 200 bp 300 bp Result and Discussion

7. f serotypec serotypee serotype PCR Documentation of f, c and e serotype Streptococcus mutans in preschool children

8. f serotype 91% (31/34) c serotype 79% (27/34) e serotype 29% (10/34) Serotype Distribution

9. Shibata (2003) in Japanese preschool children Serotype c 84.8% Serotype e 13.3% Serotype f 1.9% Nakano (2007) Non-c serotypes was higher in cardiovascular subjects while serotype c was dominant in healthy subjects

10. Norio Masuda (1979) c serotype was dominant for children between 5 to 13 months Hölttä (1994) The distribution of mutans streptococci serotypes in Finnish and Vietnamese children was: c serotype 100% (Finnish); 50% (Vietnamese) e serotype10% (Finnish); 31% (Vietnamese) d serotype 0% (Finnish); 56% (Vietnamese) g serotype20% (Finnish); 38% (Vietnamese)

11. L. Grönroos (1998) 29 subjects serotype c isolates, 7 subject serotype e isolates, 8 subject serotype g isolates 1 subject serotype f isolates.

12. 60% f and c combination 4.3% f and e combination 23% f, c, and e combination 34.8% single serotype Serotype Combination

13. L. Grönroos (1998) 67% one serotype only 30% two serotypes 3% three serotypes Hölttä (1994) The frequency of plural serotypes between Finnish/Vietnamese children was 30%/75%

14. The difference results could be: method for serotyping Geographical differences Age of subjects Diet consumption

15. Conclusion f serotype was a dominant serotype founded in children observed even thought some of them have serotype combination with c and e.

16. Future Works Saliva protein will be studied in baby bottle caries

17. Acknowledgement This study was supported by RUUI research grant programme 2006