1. Inner-Cell-Mass Cell Embryonic Stem Cells Ectoderm Mesoderm Endoderm Blastocyst Forms Placenta Becomes Baby 3 Primary Tissue Types

2. Primary Tissue Layers Ectoderm skin nerves eyes Mesoderm bones muscles blood Endoderm Lining of gut lungsliver There are over 200 cell types in the adult body

3. Mesenchymal Stem Cells Muscle Repair of cardiac tissue Skin Skin grafts after burn or Reconstructive surgery Fat Cells Reconstruction Surgeries Nerves Repair of nerve damage Tendons / Cartilage Joint & Back Repair Bone Bone regrowth Retinal Photoreceptors Corneal Repair Regain sight after injury Teeth Repair or Replace Teeth Liver Liver Disease Insulin secreting cells Diabetes Trachea & Lung Trachea Replacement Lung Diseases Blood Vessels Tissue Repair and Regrowth Mesenchymal Stem Cells

4. Potential of MSC’s from Teeth What can these cells do functionally? There are several papers which have shown the ability of the MSC’s from teeth both the lab and in animal to differentiate down these pathways. SHED: stem cells from human exfoliated deciduous teeth. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, Shi S. Proc Natl Acad Sci U S A. 2003 May 13;100(10):5807-12. NeurogenicOsteogenicOdontogenicMyogenicChondrogenic Adipogenesis or fat production

5. Differentiation Process