1. Week 1: Orientation Orientation Biological safety Chemical safety
Fire safety
Lab glassware
Pipetting

2. MEDT 251 Goals Schedule Faculty Policies NiceNet class
Objectives: cognitive, psychomotor, affective
Offices and faculty
Resources

3. Pipets Transfer fluids Make dilutions
Make primary and secondary standards
Reconstitute reagents and controls

4. Why so many kinds?

5. Pipet Variation… Types of fluid Degree of accuracy needed
Blood, plasma, serum
Aqueous reagents
Viscosity
Degree of accuracy needed
Accuracy vs. efficiency
Amount of fluid

6. Markings on Pipet Total volume and increments
Calibration temperature: 20oC
TD: to deliver
TC: to contain
Tolerance limits

8. Etched band means “blow out”
Color bands just indicate size

9. General Types of Pipet Volumetric: for accuracy
Volumetric transfer
Ostwald
Graduated: for efficiency
Serological
Mohr

14. Pipetting Select the right pipet Check for broken tip
Aspirate with bulb
Wipe the tip
Adjust meniscus
Deliver along side of vessel
Place in wash receptacle

15. Checking…

16. No!

17. Tip is completely in the fluid
Wipe the tip after aspiration

19. Dispense fluid along side of vessel

20. Division Safety Rules Read the Division’s safety manual
Sign the Assumption of Risk and Release form