1. Healthy ControlCase 2D gel images of Serum protein sample Set of gel images for analysis

2. Steps followed in gel analysis 1) Set up Project 2) Detect and Quantify Spots 3) Analyze Expression Profiles 4) Present Results

3. ANALYSIS, RESULT AND DATA INTERPRETATION

4. Project Name Serum sample 2D-Gels for Analysis. Project Creation Date ………………. Author ………………. Description Healthy vs diseased patient Use internal Standard No Pool C\Users\XYZ Groups Control(1 image) Control - Healthy person 349spots transferred from fused image 97 spots cancelled Diseased (1 image) P.falciparum diseased serum sample 349 spots transferred from fused image 97 spots cancelled Vector Matching : 109 1. Project Summary:

5. Profile Spot Information Control Diseased Spot ID : 1999 pI : 5.26 MW : 65.1kDal 3D view Spot ID : 2082 pI : 5.28 MW : 44.8kDal 3D view Control P.fal Fused 3D view 1. Project Summary:

6. 4.004.254.504.755.005.255.505.756.006.256.506.757.00 80 75 70 65 60 55 50 45 40 35 30 25 20 15 10 5 pI index Molecular weight (kDal) 85 Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal Spot ID: 1999 pI : 5.26 Mol. weight : 65.2 kDal 3.Figure showing Molecular weight to pI

7. No. of spots showing up regulation Up regulation No. of folds (and above)No.of spots 269 347 439 533 821 1016 15 2010 258 503 751 Up regulation x Folds or above 4.1. Differential expression : Up regulation

8. ABCDEF GHIJK Mean normalized value of group-Control(Healthy person) Mean normalized value of group 2 (P.falciparum diseased person) Ratio = 4.2. expression profile: Up regulation(8-10 folds up) -11 spots

9. Down Regulation No. of folds (and above)No. of spots 283 348 437 528 713 1011 155 302 No. of spots showing up regulation Down regulation x Folds or above 4.3.Differential expression : Down regulation

10. 4.4.Expression profile: Down regulation (9-10 folds down) - 9 spots ABCDE FGHI Mean normalized value of group-Control(Healthy person) Ratio = Mean normalized value of group 2 (P.falciparum diseased person)

11. Hierarchical Cluster AnalysisHCL Tree It provides information about similarly expressed protein. It helps in finding correlated protein 5.Cluster Analysis

12. 6. Scatter plot