1. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Chapter 11 Modifying Organisms: Transgenics © 2012 John Wiley & Sons, Ltd.

2. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.1 Differential expression of beta-globins during development.

3. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.2 Two methods for producing transgenic mice.

4. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.3 Embryonic stem cell technology.

5. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.4 Gene insertion at a specific site.

6. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.5 Gene knockouts. The neo gene codes for an aminoglycoside phosphotransferase that gives resistance to neomycin and to G418 (Geneticin).

7. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.6 Construction of recombinant vaccinia virus. The plasmid carries an interrupted vaccinia TK gene. Recombination occurs between the TK sequences on the plasmid and vaccinia DNA, after co-infecting animal cells. The resulting recombinant is TK−, and expresses the insert gene from the vaccinia promoter.

8. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.7 Cloning in plant cells using a Ti plasmid.

9. From Genes to Genomes: Concepts and Applications of DNA Technology, Jeremy W. Dale, Malcolm von Schantz and Nick Plant. © 2012 John Wiley & Sons, Ltd. Figure 11.8 Gene subtraction in plants, using antisense RNA.