1. BDNF Induces Late-phase LTP of C-fiber Evoked Field Potentials in Rat Spinal Dorsal Horn

2. “It is easier to find men who will volunteer to die, than to find those who are willing to endure pain with patience.” ----Julius Caesar

3. Are We Lost? Background – LTP and BDNF Background Some More – Anatomy and Pain Results and Interpretations – THE Paper Concluding Conclusions

4. LTP: It Potentiates…Or Does It? J/K, It Does. Bliss and Lomo, 1973

5. It’s Never Too Early to be Late Early phase (E-LTP) – Independent of protein synthesis – Lasts up to 3 hours Late phase (L-LTP) – Protein synthesis involved – Lasts longer than 3 hours, up to entire life SUPER DUPER EXTREMELY SUBJECTIVE!!

6. LTP is the BMOC aka The Leo Da Vinci of Neuroscience

7. Hi, My Name Is: BDNF X

8. Hi, My Name Is: BDNF

12. Let’s Learn Anatomy!

13. Dorsal root and rootlets Spinal (dorsal root) ganglion Ventral root and rootlets Spinal nerve Netter 169 Dorsal ramus Ventral ramus Anatomy is FUN!

14. It’s All Fun and Games Until Someone Gets Their Face Burned Off Nociception: detection of pain – 2 types: mechanical and polymodal Different signaling molecules Different molecules enhance response Principles of Neural Science

15. It’s All Fun and Games Until Someone Gets Their Face Burned Off Principles of Neural Science

16. It’s All Fun and Games Until Someone Gets Their Face Burned Off Central sensitization Two main pathological pain states – Hyperalgesia – Allodynia

17. LTP Makes it Painful Sandkuhler, J., Molecular Pain.

18. Sometimes, a Little Doesn’t Go a Long Way Pezet, S. et al., Brain Research Reviews.

19. All’s I Need is a Match, a Pen, and a Compass Adult male Sprague-Dawley rats Left sciatic or sural nerve was dissected – Utilized hook electrodes for stimulation – Field potentials recorded – Both HFS and LFS

20. Um…I Might Need a Map Spared nerve injury (SNI) – Peroneal and tibial nerves cut Mechanical sensitivity through von frey hairs Drugs!!

21. Look at All the Pretty Squiggly Lines and Dots!

22. Meh.

23. I Was in Track in High School…As the Waterboy

24. “You Got Your Freshmen, ROTC Guys, Preps, J.V. Jocks, Asian Nerds, Cool Asians, Varsity Jocks, Unfriendly Black Hotties, Girls Who Eat Their Feelings, Girls Who Don't Eat Anything, Desperate Wannabes, Burnouts, Sexually Active Band Geeks, the Greatest People You Will Ever Meet, and the Worst. Beware of Plastics.”

25. Last, But Not Least

26. What Color is Your Mood? rhBDNF  LTP in spinal dorsal horn C-fibers Blocking TrkB affected BDNF- and LFS-induced LTP – BDNF differentially involved in LTP stim patterns? Inhibiting protein synthesis prevented BDNF-induced LTP

27. What is Your Motivation? BDNF-LTP NMDA-dependent BDNF occluded further potentiation in L-LTP Blocking NF-kB prevented BDNF-LTP Inhibiting ERK and p38 MAPK  no BDNF-LTP – P38 MAPK and JNK prevents LTP in injured rats Needed for initiation and maintenance of neuropathic pain in DH

28. Don’t Forget to Tip Your Waitress