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Multipotential Differentiation of Human Anulus Fibrosus Cells by Gang Feng, Xinlin Yang, Hulan Shang, Ian W. Marks, Francis H. Shen, Adam Katz, Vincent.

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Presentation on theme: "Multipotential Differentiation of Human Anulus Fibrosus Cells by Gang Feng, Xinlin Yang, Hulan Shang, Ian W. Marks, Francis H. Shen, Adam Katz, Vincent."— Presentation transcript:

1 Multipotential Differentiation of Human Anulus Fibrosus Cells by Gang Feng, Xinlin Yang, Hulan Shang, Ian W. Marks, Francis H. Shen, Adam Katz, Vincent Arlet, Cato T. Laurencin, and Xudong Li J Bone Joint Surg Am Volume 92(3):675-685 March 1, 2010 ©2010 by The Journal of Bone and Joint Surgery, Inc.

2 Representative flow cytometry analysis of the expression of cell markers in cultured anulus fibrosus cells isolated from a human intervertebral disc. Gang Feng et al. J Bone Joint Surg Am 2010;92:675-685 ©2010 by The Journal of Bone and Joint Surgery, Inc.

3 Adipogenic differentiation of cultured human anulus fibrosus cells was induced with use of the commercial adipogenic supplements in five samples. Gang Feng et al. J Bone Joint Surg Am 2010;92:675-685 ©2010 by The Journal of Bone and Joint Surgery, Inc.

4 Osteogenic differentiation of cultured human anulus fibrosus cells was induced with use of the osteogenic supplements. Gang Feng et al. J Bone Joint Surg Am 2010;92:675-685 ©2010 by The Journal of Bone and Joint Surgery, Inc.

5 Chondrogenic differentiation of human anulus fibrosus cells treated with the chondrogenic supplements. Gang Feng et al. J Bone Joint Surg Am 2010;92:675-685 ©2010 by The Journal of Bone and Joint Surgery, Inc.

6 Neurogenic differentiation of human anulus fibrosus cells by a sphere culture method with use of the NeuroCult NS-A Proliferation Kit and NeuroCult NS-A Differentiation Kit. Gang Feng et al. J Bone Joint Surg Am 2010;92:675-685 ©2010 by The Journal of Bone and Joint Surgery, Inc.

7 Endothelial lineage differentiation of cultured human anulus fibrosus cells. Gang Feng et al. J Bone Joint Surg Am 2010;92:675-685 ©2010 by The Journal of Bone and Joint Surgery, Inc.

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